Overview

  • Product name
    Anti-Mannose Receptor antibody [15-2]
    See all Mannose Receptor primary antibodies
  • Description
    Mouse monoclonal [15-2] to Mannose Receptor
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, WB, IHC-R, IHC-FoFr, Inhibition Assay, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Horse, Human
  • Immunogen

    Purified human mannose receptor from human placental tissue.

  • Epitope
    Stains extracellular domain of Mannose Receptor.
  • Positive control
    • Macrophages IHC: liver endothelial cells and Kupffer cells, spermatids, spermatozoa
  • General notes

    The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    0.2 µm filtered antibody solution
  • Primary antibody notes
    The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.
  • Clonality
    Monoclonal
  • Clone number
    15-2
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr 1/50.
WB 1/50. Detects a band of approximately 175 kDa (predicted molecular weight: 166 kDa). This antibody can be use for ligand-receptor intervention studies.
IHC-R Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration. Antibody 15-2 functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
    • Sequence similarities
      Contains 8 C-type lectin domains.
      Contains 1 fibronectin type-II domain.
      Contains 1 ricin B-type lectin domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA541I19.1 antibody
      • C type lectin domain family 13 member D antibody
      • C-type lectin domain family 13 member D antibody
      • CD 206 antibody
      • CD206 antibody
      • CD206 antigen antibody
      • CLEC13D antibody
      • CLEC13DL antibody
      • Macrophage mannose receptor 1 antibody
      • Macrophage mannose receptor 1 like protein 1 antibody
      • Macrophage mannose receptor antibody
      • Mannose receptor C type 1 antibody
      • Mannose receptor C type 1 like 1 antibody
      • MMR antibody
      • MRC 1 antibody
      • MRC1 antibody
      • MRC1_HUMAN antibody
      • MRC1L1 antibody
      • OTTHUMP00000045206 antibody
      see all

    Images

    • Frozen sections of human tonsil. ab8918 was used in a dilution of 1/25 and shows staining of endothelia of lymph vessels.

    • ab8918 staining Mannose Receptor in human mammary gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 70% Ethanol and blocking for 10 minutes at RT was performed. The sample was incubated with primary antibody (1/10) for 1 hour. A HRP-conjugated mouse polyclonal to mouse IgG was used undiluted as secondary antibody.

      See Abreview

    • ab8918 staining the Mannose Receptor in Human nasal polyp and tonsil tissue sections by Immunohistochemistry (resin-embedded sections). The tissue was fixed in acetone and inhibitors and embedded into GMA resin. Samples were incubated with primary antibody (1/400 in Tris buffered saline) for 18 hours at 21°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of murine uterus tissue sections, staining Mannose Receptor with ab8918.

      Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/40 in 1% BSA) for 1.5 hours. An undiluted HRP-conjugated rabbit anti-mouse polyclonal IgG was used as the secondary antibody.

      See Abreview

    • ab8918 staining Mannose Receptor in human endometriosis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/10 in PBS + 1% NGS) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:
    • Boulding T  et al. LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer. Sci Rep 8:73 (2018). Read more (PubMed: 29311580) »
    • Hamasaki S  et al. Effects of scavenger receptors-1 class A stimulation on macrophage morphology and highly modified advanced glycation end product-protein phagocytosis. Sci Rep 8:5901 (2018). Read more (PubMed: 29651042) »
    See all 31 Publications for this product

    Customer reviews and Q&As

    1-10 of 22 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Sample
    Human Tissue sections (inflamed human colon)
    Specification
    inflamed human colon
    Permeabilization
    No
    Fixative
    Acetone

    Abcam user community

    Verified customer

    Submitted Dec 01 2014

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample
    Human Tissue sections (Endometriosis)
    Specification
    Endometriosis
    Permeabilization
    No
    Fixative
    Acetone

    Mrs. Aimée-Lys Rusesabagina

    Verified customer

    Submitted Sep 12 2014

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Sample
    Mouse Tissue sections (Breast tumor)
    Specification
    Breast tumor
    Permeabilization
    No
    Fixative
    Paraformaldehyde

    Dr. Gajendra Katara

    Verified customer

    Submitted Sep 05 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Sample
    Mouse Cell (Peritoneal macrophages)
    Specification
    Peritoneal macrophages
    Permeabilization
    No
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Aug 20 2014

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3 · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample
    Mouse Tissue sections (Uterus)
    Specification
    Uterus
    Permeabilization
    No
    Fixative
    Paraformaldehyde

    Dr. Mukesh Jaiswal

    Verified customer

    Submitted Nov 12 2013

    Answer

    Thank you for contacting us.

    Both of these product are tested and guaranteed to work in Flow Cytometry. These are specific against extracellular domain so the permeabilization step is optional; the antibodies would be able to stain the cells without permeabilization.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Uterus)
    Specification
    Uterus
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

    Dr. Mukesh Jaiswal

    Verified customer

    Submitted Sep 21 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (placenta)
    Specification
    placenta
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 3% · Temperature: 25°C

    Dr. Varkha Agrawal

    Verified customer

    Submitted Sep 18 2012

    Answer

    Thank you very much for your interest in our antibodies.

    To our knowledge, ab8918 and ab64693have not been tested in ELISA and also not in sandwichELISA.

    The epitope of ab8918 was not mapped and the same is true for ab117644.

    I would recommend to use ab117644 as capture antibody since it is tested for ELISA.

    Therefore, I can offer a discount off a future purchase if you buy ab8918or ab64693 now, test it inELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of the tested antibody off a future order.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and testab8918or ab64693 in ELISA. I will then send a discount code. This code must be issued before purchasing ab8918or ab64693 so please wait for my reply before ordering.

    2. Purchase ab8918or ab64693 either by phone, fax, or online (www.abcam.com).

    3. Test it in ELISA.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab8918or ab64693 turns out to be unsuitable for ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you for contacting us. I am very glad to hear that you will be using our products in your experiments. I can gladly recommend three biotin labeled antibodies to suit your needs and have placed links for biotin labeled antibodies below. I would like to offer a second option which will allow you to perform these experiments but will allow you future flexibility as well. I recommend purchase of unlabeled secondary antibodies and labeling these with the marker of your choice (biotin, fluorochrome) using our EasyLink direct conjugation kits. In this way you will be able to perform serial stains using a single primary/secondary combination and will be able to optimize and trust them in this assay. You will also have the ability to use these same antibodies, apply different labels and use them in different assays such as a double or triple staining with fluorescent tags, western blot with HRP or other assays. Our EasyLink antibody conjugation kits provide asimple and quick linking process whichis part of a new generation of conjugation procedures. These eliminate many of the difficulties which were common in previous generation of conjugation kits andallow a quick and easy process to conjugate an antibody of your choice, in whatever function you need. It has the advantage of being aone-stepantibody labeling method withno separation steps. Further information about our EasyLink kits may be found at the following links: Landing Page:https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink FAQs:https://www.abcam.com/index.html?pageconfig=resource&rid=13156 The unconjugated antibodies which I would recommend would be: ab98800, anti-mouse, unconjugated:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-pre-adsorbed-ab98800.html ab98383, anti-rat, unconjugated:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-pre-adsorbed-ab98383.html ab5738, anti-Armenian hamster, unconjugated:https://www.abcam.com/Armenian-Hamster-IgG-secondary-antibody-H-L-ab5738.html For your initial biotin labeling I would recommend one of the following EasyLink Biotin (type A) labeling kits: 3 x10ug size:https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-10%C2%B5g-Biotin-ab102865.html 3 x 100ug size:https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100%C2%B5g-Biotin-ab102864.html The pre-conjugated biotin labeled antibodies can be found at the following links: ab98802 anti-mouse, biotin:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-pre-adsorbed-ab98802.html ab98414 anti-rat, biotin:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-Biotin-pre-adsorbed-ab98414.html ab5744 anti-Armenian hamster, biotin:https://www.abcam.com/Armenian-Hamster-IgG-secondary-antibody-H-L-ab5744.html Finally, as the anti-mannose receptor antibody has not yet been tested in IHC-P, I would like to offer to you enrollment in our 100% Abreview testing discount program. DISCOUNT CODE: XXXXXXX Expiration date: XXXXX There is no obligation for you to participate in this program; however should you wish to participate this code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for IHC-P with ab8918 and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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    1-10 of 22 Abreviews or Q&A

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