Overview

  • Product name
    Anti-Mannose Receptor antibody [15-2]
    See all Mannose Receptor primary antibodies
  • Description
    Mouse monoclonal [15-2] to Mannose Receptor
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, WB, IHC-R, IHC-FoFr, Inhibition Assay, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Horse, Human
  • Immunogen

    Purified human mannose receptor from human placental tissue.

  • Epitope
    Stains extracellular domain of Mannose Receptor.
  • Positive control
    • Macrophages IHC: liver endothelial cells and Kupffer cells, spermatids, spermatozoa
  • General notes

    The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    0.2 µm filtered antibody solution
  • Primary antibody notes
    The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.
  • Clonality
    Monoclonal
  • Clone number
    15-2
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr 1/50.
WB 1/50. Detects a band of approximately 175 kDa (predicted molecular weight: 166 kDa). This antibody can be use for ligand-receptor intervention studies.
IHC-R Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration. Antibody 15-2 functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
    • Sequence similarities
      Contains 8 C-type lectin domains.
      Contains 1 fibronectin type-II domain.
      Contains 1 ricin B-type lectin domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA541I19.1 antibody
      • C type lectin domain family 13 member D antibody
      • C-type lectin domain family 13 member D antibody
      • CD 206 antibody
      • CD206 antibody
      • CD206 antigen antibody
      • CLEC13D antibody
      • CLEC13DL antibody
      • Macrophage mannose receptor 1 antibody
      • Macrophage mannose receptor 1 like protein 1 antibody
      • Macrophage mannose receptor antibody
      • Mannose receptor C type 1 antibody
      • Mannose receptor C type 1 like 1 antibody
      • MMR antibody
      • MRC 1 antibody
      • MRC1 antibody
      • MRC1_HUMAN antibody
      • MRC1L1 antibody
      • OTTHUMP00000045206 antibody
      see all

    Images

    • Frozen sections of human tonsil. ab8918 was used in a dilution of 1/25 and shows staining of endothelia of lymph vessels.

    • ab8918 staining Mannose Receptor in human mammary gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 70% Ethanol and blocking for 10 minutes at RT was performed. The sample was incubated with primary antibody (1/10) for 1 hour. A HRP-conjugated mouse polyclonal to mouse IgG was used undiluted as secondary antibody.

      See Abreview

    • ab8918 staining the Mannose Receptor in Human nasal polyp and tonsil tissue sections by Immunohistochemistry (resin-embedded sections). The tissue was fixed in acetone and inhibitors and embedded into GMA resin. Samples were incubated with primary antibody (1/400 in Tris buffered saline) for 18 hours at 21°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of murine uterus tissue sections, staining Mannose Receptor with ab8918.

      Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/40 in 1% BSA) for 1.5 hours. An undiluted HRP-conjugated rabbit anti-mouse polyclonal IgG was used as the secondary antibody.

      See Abreview

    • ab8918 staining Mannose Receptor in human endometriosis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/10 in PBS + 1% NGS) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:
    • Ni H  et al. Exosomes Derived From Bone Mesenchymal Stem Cells Ameliorate Early Inflammatory Responses Following Traumatic Brain Injury. Front Neurosci 13:14 (2019). Read more (PubMed: 30733666) »
    • Nakazawa KR  et al. Accumulation and localization of macrophage phenotypes with human intervertebral disc degeneration. Spine J 18:343-356 (2018). Read more (PubMed: 29031872) »
    See all 39 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

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    1-10 of 10 Abreviews

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Sample
    Human Tissue sections (inflamed human colon)
    Specification
    inflamed human colon
    Permeabilization
    No
    Fixative
    Acetone

    Abcam user community

    Verified customer

    Submitted Dec 01 2014

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample
    Human Tissue sections (Endometriosis)
    Specification
    Endometriosis
    Permeabilization
    No
    Fixative
    Acetone

    Mrs. Aimée-Lys Rusesabagina

    Verified customer

    Submitted Sep 12 2014

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Sample
    Mouse Tissue sections (Breast tumor)
    Specification
    Breast tumor
    Permeabilization
    No
    Fixative
    Paraformaldehyde

    Dr. Gajendra Katara

    Verified customer

    Submitted Sep 05 2014

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Sample
    Mouse Cell (Peritoneal macrophages)
    Specification
    Peritoneal macrophages
    Permeabilization
    No
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Aug 20 2014

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3 · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Sample
    Mouse Tissue sections (Uterus)
    Specification
    Uterus
    Permeabilization
    No
    Fixative
    Paraformaldehyde

    Dr. Mukesh Jaiswal

    Verified customer

    Submitted Nov 12 2013

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Uterus)
    Specification
    Uterus
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

    Dr. Mukesh Jaiswal

    Verified customer

    Submitted Sep 21 2012

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (placenta)
    Specification
    placenta
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 3% · Temperature: 25°C

    Dr. Varkha Agrawal

    Verified customer

    Submitted Sep 18 2012

    Application
    Immunohistochemistry (Resin sections)
    Sample
    Human Tissue sections (Nasal polyp, tonsil and bronchial biopsies.)
    Specification
    Nasal polyp, tonsil and bronchial biopsies.

    Abcam user community

    Verified customer

    Submitted Sep 28 2010

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Mammary gland)
    Specification
    Mammary gland
    Fixative
    70% Ethanol
    Permeabilization
    No
    Blocking step
    Protein Block (Dako) as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: RT°C

    Dr. Traci Lyons

    Verified customer

    Submitted Jul 13 2009

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Mammary gland)
    Specification
    Mammary gland
    Fixative
    Neutral Buffered Formalin
    Antigen retrieval step
    Other - Buffer/Enzyme Used: Sodium Citrate, EDTA
    Permeabilization
    Yes - Prot K
    Blocking step
    Dual endogenous, Protein Block as blocking agent for 10 minute(s) · Concentration: 100%

    Abcam user community

    Verified customer

    Submitted Jul 13 2009

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