Immunocytochemistry/ Immunofluorescence abreview for Anti-Mannose Receptor antibody [15-2]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Mouse Cell (Peritoneal macrophages)
Specification
Peritoneal macrophages
Permeabilization
No
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% FBS
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Invitrogen Donkey anti-Mouse Antibody
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Dilution
1/200

Additional data

Additional Notes
The reviewer confirmed that Fc receptors were blocked and that an isotype control was negative.

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Submitted Aug 20 2014

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