Anti-Mannose Receptor antibody (ab64693)
- Datasheet
- References (121)
- Protocols
Overview
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Product nameAnti-Mannose Receptor antibody
See all Mannose Receptor primary antibodies -
DescriptionRabbit polyclonal to Mannose Receptor
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Host speciesRabbit
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Specificity
We obtained a weaker signal with mouse samples than with human samples in Western blot when the same amount of protein was used.
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Tested applicationsSuitable for: IHC-P, WB, IHC-Fr, Flow Cyt, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Rhesus monkey
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1400 to the C-terminus of Human Mannose Receptor.
Read Abcam's proprietary immunogen policy (Peptide available as ab66694.) -
Positive control
- This antibody gave a positive signal with Rat Liver Tissue Lysate and in FFPE human lung tissue sections.
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General notes
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Alternative Products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab64693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. We do not recommend methanol fixation with this antibody |
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| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 166 kDa). | |
| IHC-Fr | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | Use a concentration of 1 µg/ml. Permeabilise with Tween (0.1%) |
Target
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FunctionMediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
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Sequence similaritiesContains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 4360 Human
- Entrez Gene: 17533 Mouse
- Entrez Gene: 291327 Rat
- Omim: 153618 Human
- SwissProt: P22897 Human
- SwissProt: Q61830 Mouse
- Unigene: 75182 Human
- Unigene: 2019 Mouse
see all -
Alternative names
- bA541I19.1 antibody
- C type lectin domain family 13 member D antibody
- C-type lectin domain family 13 member D antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)IHC image of ab64693 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64693, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Western blot - Anti-Mannose Receptor antibody (ab64693)Anti-Mannose Receptor antibody (ab64693) at 1 µg/ml + Liver (Rat) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody (ab64693)ICC/IF image of ab64693 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64693, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, HepG2, Hek293 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, MCF-7 cells at 5µg/ml. -
Western blot - Anti-Mannose Receptor antibody (ab64693)All lanes : ab64693 at 10 µg
Lane 1 : Liver (Rat) Tissue Lysate at 10 µg
Lane 2 : Lung (Human) tissue lysate at 10 µg
Lane 3 : Lung (Rat) tissue lysate at 10 µg
Lane 4 : Lung (Mouse) tissue lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa (possible non-specific binding), 50 kDa (possible non-specific binding), 56 kDa (possible non-specific binding)
Exposure time: 4 minutes -
Immunohistochemistry (Frozen sections) - Anti-Mannose Receptor antibody (ab64693)This image is courtesy of an anonymous Abreviewab64693 staining Mannose Receptor in Mouse heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 0.075% H2O2 for 10 minutes at room temperature. Samples were incubated with primary antibody (1/100 in 1x PBS + 10% heat inactivated FCS) for 1 hour. An undiluted HRP-conjugated Rabbit anti-rabbit IgG polyclonal was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Stafeev I et al. Low proliferative potential of adipose-derived stromal cells associates with hypertrophy and inflammation in subcutaneous and omental adipose tissue of patients with type 2 diabetes mellitus. J Diabetes Complications 33:148-159 (2019). Read more (PubMed: 30482492) »
- Fusser M et al. Cabazitaxel-loaded Poly(2-ethylbutyl cyanoacrylate) nanoparticles improve treatment efficacy in a patient derived breast cancer xenograft. J Control Release 293:183-192 (2019). Read more (PubMed: 30529259) »
