Anti-Mannose Receptor antibody (ab64693)

Rabbit polyclonal Mannose Receptor antibody. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human. Cited in 121 publication(s). Independently reviewed in 24 review(s).

Overview

  • Product name
    Anti-Mannose Receptor antibody
    See all Mannose Receptor primary antibodies
  • Description
    Rabbit polyclonal to Mannose Receptor
  • Host species
    Rabbit
  • Specificity

    We obtained a weaker signal with mouse samples than with human samples in Western blot when the same amount of protein was used.  

  • Tested applications
    Suitable for: IHC-P, WB, IHC-Fr, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1400 to the C-terminus of Human Mannose Receptor.

    Read Abcam's proprietary immunogen policy (Peptide available as ab66694.)

  • Positive control
    • This antibody gave a positive signal with Rat Liver Tissue Lysate and in FFPE human lung tissue sections. ICC/IF: MOLT4 cell line.
  • General notes

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

We do not recommend methanol fixation with this antibody

WB Use a concentration of 1 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 166 kDa).
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.

Permeabilise with Tween (0.1%)

Target

  • Function
    Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
  • Sequence similarities
    Contains 8 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA541I19.1 antibody
    • C type lectin domain family 13 member D antibody
    • C-type lectin domain family 13 member D antibody
    • CD 206 antibody
    • CD206 antibody
    • CD206 antigen antibody
    • CLEC13D antibody
    • CLEC13DL antibody
    • Macrophage mannose receptor 1 antibody
    • Macrophage mannose receptor 1 like protein 1 antibody
    • Macrophage mannose receptor antibody
    • Mannose receptor C type 1 antibody
    • Mannose receptor C type 1 like 1 antibody
    • MMR antibody
    • MRC 1 antibody
    • MRC1 antibody
    • MRC1_HUMAN antibody
    • MRC1L1 antibody
    • OTTHUMP00000045206 antibody
    see all

Images

  • IHC image of ab64693 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64693, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-Mannose Receptor antibody (ab64693) at 1 µg/ml + Liver (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 166 kDa
    Observed band size: 190 kDa
    why is the actual band size different from the predicted?

  • ab64693 staining Mannose Receptor in MOLT4 cells. The cells were fixed with 100% methanol (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions.  The cells were then incubated overnight at +4°C with ab64693 at 1ugml then detected with an Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150081) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue), and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red).

  • All lanes : ab64693 at 10 µg

    Lane 1 : Liver (Rat) Tissue Lysate at 10 µg
    Lane 2 : Lung (Human) tissue lysate at 10 µg
    Lane 3 : Lung (Rat) tissue lysate at 10 µg
    Lane 4 : Lung (Mouse) tissue lysate

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 166 kDa
    Observed band size: 190 kDa why is the actual band size different from the predicted?
    Additional bands at: 41 kDa (possible non-specific binding), 50 kDa (possible non-specific binding), 56 kDa (possible non-specific binding)


    Exposure time: 4 minutes
  • ab64693 staining Mannose Receptor in Mouse heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 0.075% H2O2 for 10 minutes at room temperature. Samples were incubated with primary antibody (1/100 in 1x PBS + 10% heat inactivated FCS) for 1 hour. An undiluted HRP-conjugated Rabbit anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Stafeev I  et al. Low proliferative potential of adipose-derived stromal cells associates with hypertrophy and inflammation in subcutaneous and omental adipose tissue of patients with type 2 diabetes mellitus. J Diabetes Complications 33:148-159 (2019). Read more (PubMed: 30482492) »
  • Fusser M  et al. Cabazitaxel-loaded Poly(2-ethylbutyl cyanoacrylate) nanoparticles improve treatment efficacy in a patient derived breast cancer xenograft. J Control Release 293:183-192 (2019). Read more (PubMed: 30529259) »
See all 124 Publications for this product

Customer reviews and Q&As

1-10 of 32 Abreviews or Q&A

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab64693.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Axolotl Tissue sections (Arm)
Permeabilization
No
Specification
Arm
Blocking step
DAKO serum free blocking as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0µg/mL · Temperature: 25°C
Fixative
N/a

Miss. Crystal Jones

Verified customer

Submitted Nov 02 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (lungs)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
lungs
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Nov 02 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lungs)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
lungs
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Oct 11 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lungs)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
lungs
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Oct 10 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Lymph Node, Spleen, Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 4
Permeabilization
No
Specification
Lymph Node, Spleen, Liver
Blocking step
Ultratek HRP (anti-polyvalent), ScyTek Laboratories, Logan, Utah, USA as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde

Sara Belluco

Verified customer

Submitted May 23 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse LLC tumor and spleen)
Permeabilization
No
Specification
Mouse LLC tumor and spleen
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Jan 29 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 28 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Acomys (spiny mouse) Tissue sections (spleen)
Specification
spleen
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Miss. Crystal Jones

Verified customer

Submitted Nov 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate/Ventana RiboCC
Permeabilization
No
Specification
Lung
Fixative
Paraformaldehyde

Dr. Li Li

Verified customer

Submitted May 16 2017

1-10 of 32 Abreviews or Q&A

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