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    mannose-receptor-antibody-ab64693.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Mannose Receptor antibody (ab64693)

  • Datasheet
  • SDS
Reviews (35)Q&A (8)References (427)

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Western blot - Anti-Mannose Receptor antibody (ab64693)
  • Immunocytochemistry - Anti-Mannose Receptor antibody (ab64693)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

Key features and details

  • Rabbit polyclonal to Mannose Receptor
  • Suitable for: IHC-P, WB, ICC
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Mannose Receptor antibody [EPR22489-7] (ab252921)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Mannose Receptor antibody
    See all Mannose Receptor primary antibodies
  • Description

    Rabbit polyclonal to Mannose Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1400 to the C-terminus of Human Mannose Receptor.

    Read Abcam's proprietary immunogen policy (Peptide available as ab66694.)
  • Positive control

    • WB: Rat liver and lung, human lung, mouse lung tissue lysate. IHC-P: Human, rat and mouse lung tissue. ICC: MOLT-4 whole cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Immunoglobulins
    • Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Mannose Receptor peptide (ab66694)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Mannose Receptor protein (ab152538)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
    • Anti-CD68 antibody [FA-11] (ab53444)
    • Anti-CD11b antibody [M1/70] (ab8878)
    • Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (21)
Use a concentration of 0.05 - 0.1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

We do not recommend methanol fixation with this antibody

WB (2)
Use a concentration of 1 µg/ml. Predicted molecular weight: 166 kDa.
ICC
Use a concentration of 1 µg/ml.

Permeabilise with Tween (0.1%)

Notes
IHC-P
Use a concentration of 0.05 - 0.1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

We do not recommend methanol fixation with this antibody

WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 166 kDa.
ICC
Use a concentration of 1 µg/ml.

Permeabilise with Tween (0.1%)

Target

  • Function

    Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
  • Sequence similarities

    Contains 8 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P22897 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4360 Human
    • Entrez Gene: 17533 Mouse
    • Entrez Gene: 291327 Rat
    • Omim: 153618 Human
    • SwissProt: P22897 Human
    • SwissProt: Q61830 Mouse
    • Unigene: 75182 Human
    • Unigene: 2019 Mouse
    • Alternative names

      • bA541I19.1 antibody
      • C type lectin domain family 13 member D antibody
      • C-type lectin domain family 13 member D antibody
      • CD 206 antibody
      • CD206 antibody
      • CD206 antigen antibody
      • CLEC13D antibody
      • CLEC13DL antibody
      • Macrophage mannose receptor 1 antibody
      • Macrophage mannose receptor 1 like protein 1 antibody
      • Macrophage mannose receptor antibody
      • Mannose receptor C type 1 antibody
      • Mannose receptor C type 1 like 1 antibody
      • MMR antibody
      • MRC 1 antibody
      • MRC1 antibody
      • MRC1_HUMAN antibody
      • MRC1L1 antibody
      • OTTHUMP00000045206 antibody
      see all

    Images

    • Western blot - Anti-Mannose Receptor antibody (ab64693)
      Western blot - Anti-Mannose Receptor antibody (ab64693)
      All lanes : Anti-Mannose Receptor antibody (ab64693) at 1 µg/ml

      Lane 1 : Rat liver tissue lysate
      Lane 2 : Human lung tissue lysate
      Lane 3 : Rat lung tissue lysate
      Lane 4 : Mouse lung normal tissue lysate - total protein (ab29297)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 166 kDa
      Observed band size: 190 kDa why is the actual band size different from the predicted?


      Exposure time: 4 minutes
    • Immunocytochemistry - Anti-Mannose Receptor antibody (ab64693)
      Immunocytochemistry - Anti-Mannose Receptor antibody (ab64693)

      ab64693 staining Mannose Receptor in MOLT4 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab64693 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

      IHC image of ab64693 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64693, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)This image is courtesy of an anonymous Abreview

      Paraffin embedded, formalin-fixed mouse lung tissue labelling the mannose receptor with ab64693 at 1/500 in immunohistochemical analysis. An Alexa Fluor® 647 Goat anti-rabbit IgG was used as the secondary antibody, this was artificially colored green by software to enhance stain visibility. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. 15% serum was used as blocking agent for 1 hour at room temperature.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

      IHC image of Mannose Receptor staining in a section of formalin-fixed paraffin-embedded normal rat lung performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab64693, 0.01ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

      IHC image of Mannose Receptor staining in a section of formalin-fixed paraffin-embedded normal mouse lung performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab64693, 0.01ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (427)

    Publishing research using ab64693? Please let us know so that we can cite the reference in this datasheet.

    ab64693 has been referenced in 427 publications.

    • Hou C  et al. Mono-macrophage-Derived MANF Protects Against Lipopolysaccharide-Induced Acute Kidney Injury via Inhibiting Inflammation and Renal M1 Macrophages. Inflammation 44:693-703 (2021). PubMed: 33145627
    • Borgonetti V  et al. Targeting the RNA-Binding Protein HuR Alleviates Neuroinflammation in Experimental Autoimmune Encephalomyelitis: Potential Therapy for Multiple Sclerosis. Neurotherapeutics 18:412-429 (2021). PubMed: 33200288
    • Galarraga-Vinueza ME  et al. Macrophage polarization in peri-implantitis lesions. Clin Oral Investig 25:2335-2344 (2021). PubMed: 32886246
    • Zhao Y  et al. Cetuximab enhances the anti-tumor function of macrophages in an IL-6 dependent manner. Life Sci 267:118953 (2021). PubMed: 33359746
    • Wu H  et al. Mer regulates microglial/macrophage M1/M2 polarization and alleviates neuroinflammation following traumatic brain injury. J Neuroinflammation 18:2 (2021). PubMed: 33402181
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 43 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannose Receptor antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    SuperBlock as blocking agent for 30 minute(s) · Concentration: 100%
    Fixative
    Methanol
    Read More

    MISS. Mette Schreurs

    Verified customer

    Submitted Sep 23 2021

    Immunohistochemistry free floating abreview for Anti-Mannose Receptor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 15 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannose Receptor antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mouse melanoma tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
    Permeabilization
    Yes - Tween
    Specification
    Mouse melanoma tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 15 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannose Receptor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lungs)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris EDTA Ph.9
    Permeabilization
    No
    Specification
    Lungs
    Blocking step
    (agent) for 12 minute(s) · Concentration: 0% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Syeda Mariam Riaz

    Verified customer

    Submitted Oct 22 2020

    Mass Cytometry abreview for Anti-Mannose Receptor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Mass Cytometry
    Sample
    Human Cell (cutaneous squamous cell carcinoma)
    Specification
    cutaneous squamous cell carcinoma
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 14 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannose Receptor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: BioCare Diva decloaker
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    MR. Victor Bernal

    Verified customer

    Submitted Aug 26 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-Mannose Receptor antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rabbit Tissue sections (Brain section)
    Permeabilization
    No
    Specification
    Brain section
    Blocking step
    NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    DR. Govindaiah Vinukonda

    Verified customer

    Submitted Aug 24 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannose Receptor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Rat heart infarction)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH6.0
    Permeabilization
    No
    Specification
    Rat heart infarction
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Marie-Élaine Clavet

    Verified customer

    Submitted Mar 20 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Mannose Receptor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (lung)
    Permeabilization
    No
    Specification
    lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 07 2020

    Question

    I have used ab64693 in the past with rat tissue sections and have had great success in my IHC-P staining. However this new batch, 59764, is not working nearly as well. Using the same concentration as the previous lot produced no staining and using a much higher concentration just produced high background.

    Read More

    Abcam community

    Verified customer

    Asked on May 01 2012

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab64693.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on May 01 2012

    1-10 of 43 Abreviews or Q&A

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