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    mannose-receptor-antibody-ab64693.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Mannose Receptor antibody (ab64693)

  • Datasheet
  • SDS
Reviews (32)Q&A (8)References (262)

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Western blot - Anti-Mannose Receptor antibody (ab64693)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
  • Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody (ab64693)

Key features and details

  • Rabbit polyclonal to Mannose Receptor
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Mannose Receptor protein (ab152538)
Primary
Product image
Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Mannose Receptor antibody
    See all Mannose Receptor primary antibodies
  • Description

    Rabbit polyclonal to Mannose Receptor
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1400 to the C-terminus of Human Mannose Receptor.

    Read Abcam's proprietary immunogen policy (Peptide available as ab66694.)
  • Positive control

    • WB: Rat liver and lung, human lung, mouse lung tissue lysate. IHC-P: Human, rat and mouse lung tissue. ICC: MOLT-4 whole cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Immunoglobulins
    • Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Mannose Receptor peptide (ab66694)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Mannose Receptor protein (ab152538)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)
    • Anti-CD68 antibody [FA-11] (ab53444)
    • Anti-CD11b antibody [M1/70] (ab8878)
    • Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Mouse
Rat
Human
WB
Rat
All applications
Chimpanzee
Rhesus monkey
Application Abreviews Notes
IHC-P (19)
Use a concentration of 0.05 - 0.1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

We do not recommend methanol fixation with this antibody

WB (2)
Use a concentration of 1 µg/ml. Predicted molecular weight: 166 kDa.
ICC/IF (1)
Use a concentration of 1 µg/ml.

Permeabilise with Tween (0.1%)

Notes
IHC-P
Use a concentration of 0.05 - 0.1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

We do not recommend methanol fixation with this antibody

WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 166 kDa.
ICC/IF
Use a concentration of 1 µg/ml.

Permeabilise with Tween (0.1%)

Target

  • Function

    Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
  • Sequence similarities

    Contains 8 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P22897 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4360 Human
    • Entrez Gene: 17533 Mouse
    • Entrez Gene: 291327 Rat
    • Omim: 153618 Human
    • SwissProt: P22897 Human
    • SwissProt: Q61830 Mouse
    • Unigene: 75182 Human
    • Unigene: 2019 Mouse
    • Unigene: 9197 Rat
    see all
  • Alternative names

    • bA541I19.1 antibody
    • C type lectin domain family 13 member D antibody
    • C-type lectin domain family 13 member D antibody
    • CD 206 antibody
    • CD206 antibody
    • CD206 antigen antibody
    • CLEC13D antibody
    • CLEC13DL antibody
    • Macrophage mannose receptor 1 antibody
    • Macrophage mannose receptor 1 like protein 1 antibody
    • Macrophage mannose receptor antibody
    • Mannose receptor C type 1 antibody
    • Mannose receptor C type 1 like 1 antibody
    • MMR antibody
    • MRC 1 antibody
    • MRC1 antibody
    • MRC1_HUMAN antibody
    • MRC1L1 antibody
    • OTTHUMP00000045206 antibody
    see all

Images

  • Western blot - Anti-Mannose Receptor antibody (ab64693)
    Western blot - Anti-Mannose Receptor antibody (ab64693)
    All lanes : Anti-Mannose Receptor antibody (ab64693) at 1 µg/ml

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Human lung tissue lysate
    Lane 3 : Rat lung tissue lysate
    Lane 4 : Mouse lung normal tissue lysate - total protein (ab29297)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 166 kDa
    Observed band size: 190 kDa why is the actual band size different from the predicted?


    Exposure time: 4 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

    IHC image of ab64693 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64693, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)This image is courtesy of an anonymous Abreview

    Paraffin embedded, formalin-fixed mouse lung tissue labelling the mannose receptor with ab64693 at 1/500 in immunohistochemical analysis. An Alexa Fluor® 647 Goat anti-rabbit IgG was used as the secondary antibody, this was artificially colored green by software to enhance stain visibility. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. 15% serum was used as blocking agent for 1 hour at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

    IHC image of Mannose Receptor staining in a section of formalin-fixed paraffin-embedded normal rat lung performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab64693, 0.01ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannose Receptor antibody (ab64693)

    IHC image of Mannose Receptor staining in a section of formalin-fixed paraffin-embedded normal mouse lung performed on a Leica BONDTM system using the standard protocol. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab64693, 0.01ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody (ab64693)
    Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody (ab64693)

    ab64693 staining Mannose Receptor in MOLT-4 (human lymphoblastic leukemia cell line) whole cells. The cells were fixed with 100% methanol (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions.  The cells were then incubated with the antibody ab64693 at 1µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (262)

Publishing research using ab64693? Please let us know so that we can cite the reference in this datasheet.

ab64693 has been referenced in 262 publications.

  • Zhang Q  et al. Apoptotic SKOV3 cells stimulate M0 macrophages to differentiate into M2 macrophages and promote the proliferation and migration of ovarian cancer cells by activating the ERK signaling pathway. Int J Mol Med 45:10-22 (2020). PubMed: 31746376
  • Zhao SJ  et al. Macrophage MSR1 promotes BMSC osteogenic differentiation and M2-like polarization by activating PI3K/AKT/GSK3ß/ß-catenin pathway. Theranostics 10:17-35 (2020). PubMed: 31903103
  • Orgaz JL  et al. Myosin II Reactivation and Cytoskeletal Remodeling as a Hallmark and a Vulnerability in Melanoma Therapy Resistance. Cancer Cell 37:85-103.e9 (2020). PubMed: 31935375
  • Zhou J  et al. LncGBP9/miR-34a axis drives macrophages toward a phenotype conducive for spinal cord injury repair via STAT1/STAT6 and SOCS3. J Neuroinflammation 17:134 (2020). PubMed: 32345320
  • Yang HC  et al. C-C chemokine receptor type 2-overexpressing exosomes alleviated experimental post-stroke cognitive impairment by enhancing microglia/macrophage M2 polarization. World J Stem Cells 12:152-167 (2020). PubMed: 32184939
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 40 Abreviews or Q&A

Question

I have used ab64693 in the past with rat tissue sections and have had great success in my IHC-P staining. However this new batch, 59764, is not working nearly as well. Using the same concentration as the previous lot produced no staining and using a much higher concentration just produced high background.

Read More

Abcam community

Verified customer

Asked on May 01 2012

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab64693.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on May 01 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannose Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lungs)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA Ph.9
Permeabilization
No
Specification
Lungs
Blocking step
(agent) for 12 minute(s) · Concentration: 0% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Syeda Mariam Riaz

Verified customer

Submitted Oct 22 2020

Mass Cytometry abreview for Anti-Mannose Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Mass Cytometry
Sample
Human Cell (cutaneous squamous cell carcinoma)
Specification
cutaneous squamous cell carcinoma
Read More

Abcam user community

Verified customer

Submitted Oct 14 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannose Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: BioCare Diva decloaker
Permeabilization
No
Specification
Spleen
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Formaldehyde
Read More

Mr. Victor Bernal

Verified customer

Submitted Aug 26 2020

Immunohistochemistry (Frozen sections) abreview for Anti-Mannose Receptor antibody

Inconclusive
Abreviews
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (Brain section)
Permeabilization
No
Specification
Brain section
Blocking step
NDS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Govindaiah Vinukonda

Verified customer

Submitted Aug 24 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannose Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat heart infarction)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6.0
Permeabilization
No
Specification
Rat heart infarction
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Marie-Élaine Clavet

Verified customer

Submitted Mar 20 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Mannose Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (lung)
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 07 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannose Receptor antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Triton
Specification
Bone
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Dr. Tina Yuan

Verified customer

Submitted Oct 24 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Mannose Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (lymph node)
Antigen retrieval step
None
Specification
lymph node
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Le Zhen

Verified customer

Submitted Sep 17 2019

Immunohistochemistry (Frozen sections) abreview for Anti-Mannose Receptor antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Axolotl Tissue sections (Arm)
Permeabilization
No
Specification
Arm
Blocking step
DAKO serum free blocking as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0µg/mL · Temperature: 25°C
Fixative
N/a
Read More

Miss. Crystal Jones

Verified customer

Submitted Nov 02 2018

1-10 of 40 Abreviews or Q&A

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