Recombinant
RabMAb

Recombinant Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)

Overview

  • Product name

    Anti-Mannose Receptor antibody [EPR6828(B)]
    See all Mannose Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR6828(B)] to Mannose Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Mannose Receptor aa 1400 to the C-terminus. The exact sequence is proprietary.
    Database link: P22897

  • Positive control

    • WB: HeLa membrane extract lysate (ab29547), BxPC3, Caco2, HepG2, Raw264.7 and 293T lysates, Human fetal liver tissue lysate, human, mouse and Rat brain. ICC/IF: MOLT-4 cells FC: Molt-4 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.75 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6828(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 166 kDa).
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
    • Sequence similarities

      Contains 8 C-type lectin domains.
      Contains 1 fibronectin type-II domain.
      Contains 1 ricin B-type lectin domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • bA541I19.1 antibody
      • C type lectin domain family 13 member D antibody
      • C-type lectin domain family 13 member D antibody
      • CD 206 antibody
      • CD206 antibody
      • CD206 antigen antibody
      • CLEC13D antibody
      • CLEC13DL antibody
      • Macrophage mannose receptor 1 antibody
      • Macrophage mannose receptor 1 like protein 1 antibody
      • Macrophage mannose receptor antibody
      • Mannose receptor C type 1 antibody
      • Mannose receptor C type 1 like 1 antibody
      • MMR antibody
      • MRC 1 antibody
      • MRC1 antibody
      • MRC1_HUMAN antibody
      • MRC1L1 antibody
      • OTTHUMP00000045206 antibody
      see all

    Images

    • Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028) at 1/1000 dilution + HEK-293 (Human embryonic kidney) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 166 kDa

    • Immunocytochemistry/Immunofluorescence analysis MOLT-4 (Human lymphoblastic leukemia) cells labeling Mannose Receptor with ab125028 at 1 μg/mL. Cells were fixed with 100% methanol. ab150081, an AlexaFluor®488 Goat anti-Rabbit at 1/1000 was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/250) - Microtubule Marker (Alexa Fluor® 594). Nuclei were counterstained with DAPI (blue).

      Confocal image showing membranous staining on MOLT-4 cell line.

    • All lanes : Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028) at 1/1000 dilution

      Lane 1 : BxPC3 lysate
      Lane 2 : Caco2 lysate
      Lane 3 : HepG2 lysate
      Lane 4 : Human fetal liver lysate
      Lane 5 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size: 166 kDa

    • Flow Cytometry analysis of Molt-4 (human acute lymphoblastic leukemia ) cells labeling Mannose Receptor with unpurified ab125028 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Keeler DM  et al. Brevenal, a Marine Natural Product, is Anti-Inflammatory and an Immunomodulator of Macrophage and Lung Epithelial Cells. Mar Drugs 17:N/A (2019). Read more (PubMed: 30897777) »
    • Li Y  et al. Bone marrow macrophage M2 polarization and adipose-derived stem cells osteogenic differentiation synergistically promote rehabilitation of bone damage. J Cell Biochem 120:19891-19901 (2019). Read more (PubMed: 31338874) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (kidney, mesangial)
    Permeabilization
    No
    Specification
    kidney, mesangial
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
    Fixative
    Paraformaldehyde

    Miss. Kara Shumansky

    Verified customer

    Submitted Mar 04 2019

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Macrophages Type 2)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    20 µg
    Specification
    Human Macrophages Type 2
    Blocking step
    Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C

    Dr. Francesco Elia Marino

    Verified customer

    Submitted Mar 21 2016

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (muscle)
    Specification
    muscle
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde

    Mr. Thomas Zwakhals

    Verified customer

    Submitted Mar 15 2016

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    Yes - 0.1 M PBS with 3% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Jun 25 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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