Recombinant Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6828(B)] to Mannose Receptor
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Mannose Receptor antibody [EPR6828(B)]
See all Mannose Receptor primary antibodies -
Description
Rabbit monoclonal [EPR6828(B)] to Mannose Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Mannose Receptor aa 1400 to the C-terminus. The exact sequence is proprietary.
Database link: P22897 -
Positive control
- WB: Human lung tissue; Flow Cyt (intra): MOLT-4 (human lymphoblastic leukemia cell line).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.75 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6828(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 166 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 166 kDa). |
Target
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Function
Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. -
Sequence similarities
Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4360 Human
- Omim: 153618 Human
- SwissProt: P22897 Human
- Unigene: 75182 Human
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Alternative names
- bA541I19.1 antibody
- C type lectin domain family 13 member D antibody
- C-type lectin domain family 13 member D antibody
see all
Images
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All lanes : Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028) at 1/1000 dilution
Lane 1 : Human lung tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lane 5 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 166 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa (possible non-specific binding), 65 kDa (possible non-specific binding)
Exposure time: 20 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed in U-937 and THP-1 is consistent with the literature (PMID: 20801507 and PMID: 30591689). Rabbit monoclonal [EPR16891] to GAPDH (ab181602) used as loading control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (42)
ab125028 has been referenced in 42 publications.
- Chen Y et al. PP1A prevents ROS-induced pyroptosis by inhibiting MAPK/caspase-3 in mouse adipose tissue. FEBS J 289:3839-3853 (2022). PubMed: 35080339
- Jia H et al. NLRP3 inflammasome inhibitor ameliorates ischemic stroke by reprogramming the phenotype of microglia/macrophage in a murine model of distal middle cerebral artery occlusion. Neuropathology 42:181-189 (2022). PubMed: 35434787
- Rogal J et al. Autologous Human Immunocompetent White Adipose Tissue-on-Chip. Adv Sci (Weinh) 9:e2104451 (2022). PubMed: 35466539
- Taglietti V et al. Duchenne muscular dystrophy trajectory in R-DMDdel52 preclinical rat model identifies COMP as biomarker of fibrosis. Acta Neuropathol Commun 10:60 (2022). PubMed: 35468843
- Yan P et al. Dapagliflozin Alleviates Coxsackievirus B3-induced Acute Viral Myocarditis by Regulating the Macrophage Polarization Through Stat3-related Pathways. Inflammation 45:2078-2090 (2022). PubMed: 35676606