Immunohistochemistry (Frozen sections) abreview for Anti-Mannose Receptor antibody [EPR6828(B)]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - 0.1 M PBS with 3% Triton X
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1M PBST with 10%donkeys serum
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa fluoro 488
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Adult rat was perfused with 4% PFA in 0.1M PBS. IFC staining was performed in brain sections with anti-Mannose receptor (Ab125028) with secondary antibody Alexa 488. The anti-Mannose receptor was visible in the blood vessels macrophages. Nucleus was stained with DAPI.

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 25 2015

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