Product nameAnti-Mannose Receptor antibody [EPR6828(B)] (HRP)
See all Mannose Receptor primary antibodies
DescriptionRabbit monoclonal [EPR6828(B)] to Mannose Receptor (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Mannose Receptor aa 1400 to the C-terminus (internal sequence). The exact sequence is proprietary.
- WB: Caco2, HEK293 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Dissociation constant (KD)KD = 1.75 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)
- Anti-Mannose Receptor antibody [EPR6828(B)] (Alexa Fluor® 488) (ab195191)
- Anti-Mannose Receptor antibody [EPR6828(B)] (Alexa Fluor® 647) (ab195192)
- Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
- Anti-Mannose Receptor antibody [EPR6828(B)] (Phycoerythrin) (ab223960)
- Anti-Mannose Receptor antibody [EPR6828(B)] (Allophycocyanin) (ab223961)
Our Abpromise guarantee covers the use of ab195193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 172 kDa (predicted molecular weight: 166 kDa).|
FunctionMediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
Sequence similaritiesContains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain.
- Information by UniProt
- bA541I19.1 antibody
- C type lectin domain family 13 member D antibody
- C-type lectin domain family 13 member D antibody
All lanes : Anti-Mannose Receptor antibody [EPR6828(B)] (HRP) (ab195193) at 1/5000 dilution
Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 166 kDa
Observed band size: 172 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195193 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab195193 has not yet been referenced specifically in any publications.