Product nameAnti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free
See all Mannose Receptor primary antibodies
DescriptionRabbit monoclonal [EPR6828(B)] to Mannose Receptor - Low endotoxin, Azide free
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Mannose Receptor aa 1400-1500 (internal sequence). The exact sequence is proprietary.
- WB: BxPC3, Caco2, HepG2, Human fetal liver and 293T lysates
ab209327 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 1.75 x 10 -10 M Learn more about KD
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
- Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)
- Anti-Mannose Receptor antibody [EPR6828(B)] (Alexa Fluor® 488) (ab195191)
- Anti-Mannose Receptor antibody [EPR6828(B)] (Alexa Fluor® 647) (ab195192)
- Anti-Mannose Receptor antibody [EPR6828(B)] (HRP) (ab195193)
- Anti-Mannose Receptor antibody [EPR6828(B)] (Phycoerythrin) (ab223960)
- Anti-Mannose Receptor antibody [EPR6828(B)] (Allophycocyanin) (ab223961)
Our Abpromise guarantee covers the use of ab209327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 170 kDa (predicted molecular weight: 166 kDa).|
|Flow Cyt||Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
FunctionMediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
Sequence similaritiesContains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain.
- Information by UniProt
- bA541I19.1 antibody
- C type lectin domain family 13 member D antibody
- C-type lectin domain family 13 member D antibody
This ICC data was generated using the same anti-Mannose Receptor antibody clone [EPR6828(B)] in a different buffer formulation (cat# ab125028).
Immunocytochemistry/Immunofluorescence analysis MOLT-4 (Human lymphoblastic leukemia) cells labeling Mannose Receptor with ab125028 at 1 μg/mL. Cells were fixed with 100% methanol. ab150081, an AlexaFluor®488 Goat anti-Rabbit at 1/1000 was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/250) - Microtubule Marker (Alexa Fluor® 594). Nuclei were counterstained with DAPI (blue).
Confocal image showing membranous staining on MOLT-4 cell line.
Flow Cytometry analysis of Molt-4 (human acute lymphoblastic leukemia ) cells labeling Mannose Receptor with unpurified ab125028 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125028).
This product has been referenced in:
- Trial J et al. Th1/M1 conversion to th2/m2 responses in models of inflammation lacking cell death stimulates maturation of monocyte precursors to fibroblasts. Front Immunol 4:287 (2013). ICC/IF ; Human . Read more (PubMed: 24065967) »