RecombinantRabMAb

Recombinant Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)

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Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
  • Flow Cytometry - Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
  • OI-RD Scanning - Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
  • Western blot - Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
  • Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6828(B)] to Mannose Receptor - Low endotoxin, Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free
    See all Mannose Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR6828(B)] to Mannose Receptor - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Mannose Receptor aa 1400-1500 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • WB: Human lung tissue; ICC/IF: MOLT-4 (human lymphoblastic leukemia cell line); Flow Cyt: MOLT-4 (human lymphoblastic leukemia cell line).

  • General notes

    ab209327 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab209327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 200 kDa (predicted molecular weight: 166 kDa).
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.

    • Sequence similarities

      Contains 8 C-type lectin domains.
      Contains 1 fibronectin type-II domain.
      Contains 1 ricin B-type lectin domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • bA541I19.1 antibody
        • C type lectin domain family 13 member D antibody
        • C-type lectin domain family 13 member D antibody
        • CD 206 antibody
        • CD206 antibody
        • CD206 antigen antibody
        • CLEC13D antibody
        • CLEC13DL antibody
        • Macrophage mannose receptor 1 antibody
        • Macrophage mannose receptor 1 like protein 1 antibody
        • Macrophage mannose receptor antibody
        • Mannose receptor C type 1 antibody
        • Mannose receptor C type 1 like 1 antibody
        • MMR antibody
        • MRC 1 antibody
        • MRC1 antibody
        • MRC1_HUMAN antibody
        • MRC1L1 antibody
        • OTTHUMP00000045206 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
        Immunocytochemistry/ Immunofluorescence - Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)

        This ICC data was generated using the same anti-Mannose Receptor antibody clone [EPR6828(B)] in a different buffer formulation (cat# ab125028).

        Immunocytochemistry/Immunofluorescence analysis MOLT-4 (Human lymphoblastic leukemia) cells labeling Mannose Receptor with ab125028 at 1 μg/mL. Cells were fixed with 100% methanol. ab150081, an AlexaFluor®488 Goat anti-Rabbit at 1/1000 was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/250) - Microtubule Marker (Alexa Fluor® 594). Nuclei were counterstained with DAPI (blue).

        Confocal image showing membranous staining on MOLT-4 cell line.

      • Flow Cytometry - Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
        Flow Cytometry - Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)

        Flow Cytometry analysis of Molt-4 (human acute lymphoblastic leukemia ) cells labeling Mannose Receptor with unpurified ab125028 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125028).

      • OI-RD Scanning - Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
        OI-RD Scanning - Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125028).

      • Western blot - Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
        Western blot - Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
        All lanes : Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028) at 1/1000 dilution

        Lane 1 : Human lung tissue lysate
        Lane 2 : Human brain tissue lysate
        Lane 3 : Mouse brain tissue lysate
        Lane 4 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
        Lane 5 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 166 kDa
        Observed band size: 200 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 160 kDa (possible non-specific binding), 65 kDa (possible non-specific binding)


        Exposure time: 20 seconds


        Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

        The expression profile observed in U-937 and THP-1 is consistent with the literature (PMID: 20801507 and PMID: 30591689). Rabbit monoclonal [EPR16891] to GAPDH (ab181602) used as loading control.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab125028).

      • Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)
        Anti-Mannose Receptor antibody [EPR6828(B)] - Low endotoxin, Azide free (ab209327)

      References (1)

      Publishing research using ab209327? Please let us know so that we can cite the reference in this datasheet.

      ab209327 has been referenced in 1 publication.

      • Trial J  et al. Th1/M1 conversion to th2/m2 responses in models of inflammation lacking cell death stimulates maturation of monocyte precursors to fibroblasts. Front Immunol 4:287 (2013). ICC/IF ; Human . PubMed: 24065967

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