- Datasheet
- Specific References (21)
- Protocols
Overview
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Product nameAnti-Mannosidase II antibody
See all Mannosidase II primary antibodies -
DescriptionRabbit polyclonal to Mannosidase II
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IFmore details
Unsuitable for: IP or WB -
Species reactivityReacts with: Human, Monkey
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide to internal sequence (Human) conjugated to KLH.
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Positive control
- ICC: COS7 cells
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium Azide
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PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab12277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/200. Use with methanol fixed cells. 4% paraformaldehyde fixation reduces signal. |
Application notes
Is unsuitable for IP or WB.
Target
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FunctionCatalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
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PathwayProtein modification; protein glycosylation.
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Sequence similaritiesBelongs to the glycosyl hydrolase 38 family.
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Cellular localizationGolgi apparatus membrane.
- Information by UniProt
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Database links
- Entrez Gene: 4124 Human
- Omim: 154582 Human
- SwissProt: Q16706 Human
- Unigene: 432822 Human
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Alternative names
- 3-1 antibody
- 6-alpha-mannosidase antibody
- Alpha mannosidase 2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Mannosidase II antibody (ab12277)COS7 cells were fixed with cold methanol for 10 minutes followed by incubation with ab12277 (1/200) in 5% normal goat serum. FITC-conjugated goat anti-rabbit was used to visualise bound rabbit antibodies. Nuclei were stained with DAPI. -
Immunocytochemistry/ Immunofluorescence - Anti-Mannosidase II antibody (ab12277)ICC/IF image of ab12277 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12277, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Jiang P et al. The Protein Encoded by the CCDC170 Breast Cancer Gene Functions to Organize the Golgi-Microtubule Network. EBioMedicine 22:28-43 (2017). Read more (PubMed: 28687497) »
- Sin AT & Harrison RE Growth of the Mammalian Golgi Apparatus during Interphase. Mol Cell Biol 36:2344-59 (2016). ICC/IF ; Human . Read more (PubMed: 27325676) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"