Anti-Mannosidase II/MAN2A1 antibody (ab77353)
Key features and details
- Goat polyclonal to Mannosidase II/MAN2A1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Mannosidase II/MAN2A1 antibody
See all Mannosidase II/MAN2A1 primary antibodies -
Description
Goat polyclonal to Mannosidase II/MAN2A1 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human Placenta lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab77353 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 131 kDa).
A 1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 131 kDa). A 1 hour primary incubation is recommended for this product. |
Target
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Function
Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyl hydrolase 38 family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 4124 Human
- Omim: 154582 Human
- SwissProt: Q16706 Human
- Unigene: 432822 Human
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Alternative names
- 3-1 antibody
- 6-alpha-mannosidase antibody
- Alpha mannosidase 2 antibody
see all
Images
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Anti-Mannosidase II/MAN2A1 antibody (ab77353) at 0.3 µg/ml + Human Placenta lysate (in RIPA buffer) at 35 µg
Predicted band size: 131 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Mannosidase II/MAN2A1 antibody (ab77353) at 3 µg/ml
Lane 1 : MOCK-transfected HEK293 cell lysate
Lane 2 : HEK293 cell lysate overexpressing Mannosidase II/MAN2A1
Predicted band size: 131 kDa
Datasheets and documents
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Datasheet download
References (0)
ab77353 has not yet been referenced specifically in any publications.