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I have two questions regarding the anti-mannosidase II antibody - Golgi marker (ab12277):
1. is suitable for FACS?
2. where is located the peptide that is recognized by the antibody? at the luminal part?
Asked on Jul 30 2012
Thank you for your questions. I apologize for the delay in replying but I was unable to contact the laboratory that produces this antibody for information about FACS testing, or for more information about the immunogen.
I expect that the antibody will be suitable for FACS, given that it has been used to stain cells for microscopy. The plasma membrane of the cells will need to be permeabilized with methanol as described in the caption of the photo of ICC/IF on the datasheet, to allow the antibody access to the mannosidase in the ER.
It is very likely that the immunogen for the antibody was derived from the lumenal region. According to the description of the human protein at the UniProt database, the cytoplasmic portion is amino acids 1-5, the transmembrane region is 5-26, and the lumenal portion is 27-1144.
Answered on Jul 30 2012