Key features and details
- Rabbit polyclonal to Mannosidase II/MAN2A1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Mannosidase II/MAN2A1 antibody
See all Mannosidase II/MAN2A1 primary antibodies
DescriptionRabbit polyclonal to Mannosidase II/MAN2A1
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
- WB: HEK-293 and HepG2 cell lysate. ICC/IF: HeLa cells. IHC-P: Human lung cancer and cervical cancer tissue.
This product was previously labelled as Mannosidase II
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab224611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000.|
FunctionCatalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyl hydrolase 38 family.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- 3-1 antibody
- 6-alpha-mannosidase antibody
- Alpha mannosidase 2 antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Mannosidase II/MAN2A1 (green) using ab224611 (1/100 dilution) in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin embedded human cervical cancer tissue stained for Mannosidase II/MAN2A1 with ab224611 (1/100 dilution) in immunohistochemical analysis.
All lanes : Anti-Mannosidase II/MAN2A1 antibody (ab224611) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Paraffin embedded human lung cancer tissue stained for Mannosidase II/MAN2A1 with ab224611 (1/100 dilution) in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224611 has not yet been referenced specifically in any publications.