Overview

  • Product name

    Anti-Mannosidase II/MAN2A1 antibody
    See all Mannosidase II/MAN2A1 primary antibodies
  • Description

    Goat polyclonal to Mannosidase II/MAN2A1
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Mannosidase II/MAN2A1 aa 881-891 (internal sequence).
    Sequence:

    KISSDIKSQNR

  • Positive control

    • Human Placenta lysate.
  • General notes

     This product was previously labelled as Mannosidase II

     

Properties

Applications

Our Abpromise guarantee covers the use of ab77353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 131 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Function

    Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyl hydrolase 38 family.
  • Cellular localization

    Golgi apparatus membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3-1 antibody
    • 6-alpha-mannosidase antibody
    • Alpha mannosidase 2 antibody
    • alpha Mannosidase II antibody
    • Alpha-mannosidase 2 antibody
    • AMAN II antibody
    • Golgi alpha mannosidase II antibody
    • Golgi alpha-mannosidase II antibody
    • Golgi integral membrane protein 7 antibody
    • GOLIM7 antibody
    • MA2A1_HUMAN antibody
    • MAN II antibody
    • Man2a1 antibody
    • MANA 2 antibody
    • MANA2 antibody
    • MANII antibody
    • Mann II antibody
    • Mannosidase alpha class 2A member 1 antibody
    • Mannosidase Two antibody
    • Mannosidase, alpha type II antibody
    • Mannosidase, alpha, II antibody
    • Mannosyl oligosaccharide 1 3 1 6 alpha mannosidase antibody
    • Mannosyl oligosaccharide 1,3 1,6 alpha mannosidase antibody
    • Mannosyl-oligosaccharide 1 antibody
    see all

Images

  • Anti-Mannosidase II/MAN2A1 antibody (ab77353) at 0.3 µg/ml + Human Placenta lysate (in RIPA buffer) at 35 µg

    Predicted band size: 131 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-Mannosidase II/MAN2A1 antibody (ab77353) at 3 µg/ml

    Lane 1 : MOCK-transfected HEK293 cell lysate
    Lane 2 : HEK293 cell lysate overexpressing Mannosidase II/MAN2A1

    Predicted band size: 131 kDa

References

ab77353 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (human dermal fibroblasts)
Loading amount
10 µg
Specification
human dermal fibroblasts
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 04 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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