Overview

  • Product name
    Anti-Map17 antibody [EPR10372]
    See all Map17 primary antibodies
  • Description
    Rabbit monoclonal [EPR10372] to Map17
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to residues in Human Map17 (UniProt Q13113).

  • Positive control
    • Human fetal kidney lysate; Human breast carcinoma and lung carcinoma tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10372
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Relevance
      MAP17 is a 17 kDa membrane-associated protein expressed abundantly in carcinomas arising from kidney, colon, lung, and breast. In normal tissues it is expressed in significant amounts in the kidney.
    • Cellular localization
      Plasma membrane
    • Database links
      • Alternative names
        • 17 kDa membrane associated protein antibody
        • DD96 antibody
        • Epithelial protein up regulated in carcinoma membrane associated protein 17 antibody
        • Membrane associated protein 17 antibody
        • PDZK1-interacting protein 1 antibody
        • Pdzk1ip1 antibody
        • RP1-18D14.5 antibody
        • SPAP antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        ab156014 showing +ve staining in Human urinary bladder transitional carcinoma.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        ab156014 showing +ve staining in Human ovarian carcinoma.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        ab156014 showing +ve staining in Human colonic adenocarcinoma.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        ab156014 showing +ve staining in Human cervical carcinoma.
      • Western blot - Anti-Map17 antibody [EPR10372] (ab156014)
        Western blot - Anti-Map17 antibody [EPR10372] (ab156014)
        Anti-Map17 antibody [EPR10372] (ab156014) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 12 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Map17 with ab156014 at 1/100 dilution.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Map17 with ab156014 at 1/100 dilution.

      References

      This product has been referenced in:
      • Chen X  et al. Elevation of MAP17 enhances the malignant behavior of cells via the Akt/mTOR pathway in hepatocellular carcinoma. Oncotarget 8:92589-92603 (2017). Read more (PubMed: 29190940) »

      See 1 Publication for this product

      Publishing research using ab156014? Please let us know so that we can cite the reference in this datasheet.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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