Anti-Map17 antibody [EPR10372] Recombinant (ab156014)

Product name

Anti-Map17 antibody [EPR10372]
See all Map17 primary antibodies
Description

Rabbit monoclonal [EPR10372] to Map17
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment corresponding to residues in Human Map17 (UniProt Q13113).
Positive control
- Human fetal kidney lysate; Human breast carcinoma and lung carcinoma tissues.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10372
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab156014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
IHC-P		1/100 - 1/250.

Application notes

Is unsuitable for ICC or IP.

Relevance

MAP17 is a 17 kDa membrane-associated protein expressed abundantly in carcinomas arising from kidney, colon, lung, and breast. In normal tissues it is expressed in significant amounts in the kidney.
Cellular localization

Plasma membrane
Database links
- Entrez Gene: 10158 Human
- Omim: 607178 Human
- SwissProt: Q13113 Human
Alternative names
- 17 kDa membrane associated protein antibody
- DD96 antibody
- Epithelial protein up regulated in carcinoma membrane associated protein 17 antibody
- Membrane associated protein 17 antibody
- PDZK1-interacting protein 1 antibody
- Pdzk1ip1 antibody
- RP1-18D14.5 antibody
- SPAP antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)

ab156014 showing +ve staining in Human urinary bladder transitional carcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)

ab156014 showing +ve staining in Human ovarian carcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)

ab156014 showing +ve staining in Human colonic adenocarcinoma.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)

ab156014 showing +ve staining in Human cervical carcinoma.
Western blot - Anti-Map17 antibody [EPR10372] (ab156014)

Anti-Map17 antibody [EPR10372] (ab156014) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 12 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Map17 with ab156014 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Map17 with ab156014 at 1/100 dilution.

Chen X et al. Elevation of MAP17 enhances the malignant behavior of cells via the Akt/mTOR pathway in hepatocellular carcinoma. Oncotarget 8:92589-92603 (2017). Read more (PubMed: 29190940) »

Publishing research using ab156014? Please let us know so that we can cite the reference in this datasheet.

Anti-Map17 antibody [EPR10372] (ab156014)