Recombinant
RabMAb

Recombinant Anti-Map17 antibody [EPR10372] (ab156014)

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Overview

  • Product name

    Anti-Map17 antibody [EPR10372]
    See all Map17 primary antibodies

  • Description

    Rabbit monoclonal [EPR10372] to Map17

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human Map17.

  • Positive control

    • Human fetal kidney lysate; Human breast carcinoma and lung carcinoma tissues.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10372

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Relevance

      MAP17 is a 17 kDa membrane-associated protein expressed abundantly in carcinomas arising from kidney, colon, lung, and breast. In normal tissues it is expressed in significant amounts in the kidney.

    • Cellular localization

      Plasma membrane

    • Database links

      • Alternative names

        • 17 kDa membrane associated protein antibody
        • DD96 antibody
        • Epithelial protein up regulated in carcinoma membrane associated protein 17 antibody
        • Membrane associated protein 17 antibody
        • PDZK1-interacting protein 1 antibody
        • Pdzk1ip1 antibody
        • RP1-18D14.5 antibody
        • SPAP antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)

        ab156014 showing +ve staining in Human urinary bladder transitional carcinoma.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)

        ab156014 showing +ve staining in Human ovarian carcinoma.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)

        ab156014 showing +ve staining in Human colonic adenocarcinoma.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)

        ab156014 showing +ve staining in Human cervical carcinoma.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-Map17 antibody [EPR10372] (ab156014)
        Western blot - Anti-Map17 antibody [EPR10372] (ab156014)
        Anti-Map17 antibody [EPR10372] (ab156014) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 12 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)

        Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Map17 with ab156014 at 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Map17 antibody [EPR10372] (ab156014)

        Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Map17 with ab156014 at 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Tampaki EC  et al. Combined Fascin-1 and MAP17 Expression in Breast Cancer Identifies Patients with High Risk for Disease Recurrence. Mol Diagn Ther 23:635-644 (2019). Read more (PubMed: 31273628) »
      • Chen X  et al. Elevation of MAP17 enhances the malignant behavior of cells via the Akt/mTOR pathway in hepatocellular carcinoma. Oncotarget 8:92589-92603 (2017). Read more (PubMed: 29190940) »
      See all 2 Publications for this product

      Publishing research using ab156014? Please let us know so that we can cite the reference in this datasheet.

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