Recombinant
RabMAb

Recombinant Anti-Map17 antibody [EPR16827] (ab199540)

Overview

  • Product name

    Anti-Map17 antibody [EPR16827]
    See all Map17 primary antibodies
  • Description

    Rabbit monoclonal [EPR16827] to Map17
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Map17 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13113

  • Positive control

    • Human fetal kidney lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16827
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 12 kDa).
IP 1/120.

Target

  • Relevance

    MAP17 is a 17 kDa membrane-associated protein expressed abundantly in carcinomas arising from kidney, colon, lung, and breast. In normal tissues it is expressed in significant amounts in the kidney.
  • Cellular localization

    Plasma membrane
  • Database links

  • Alternative names

    • 17 kDa membrane associated protein antibody
    • DD96 antibody
    • Epithelial protein up regulated in carcinoma membrane associated protein 17 antibody
    • Membrane associated protein 17 antibody
    • PDZK1-interacting protein 1 antibody
    • Pdzk1ip1 antibody
    • RP1-18D14.5 antibody
    • SPAP antibody
    see all

Images

  • All lanes : Anti-Map17 antibody [EPR16827] (ab199540) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 12 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature PMID: 10951271.

    Human fetal brain, Human fetal heart, Human fetal spleen represent negative controls for Map17.

  • Map17 was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab199540 at 1/120 dilution. Western blot was performed from the immunoprecipitate using ab199540 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal kidney whole cell lysate 10ug (Input). Lane 2: ab199540 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199540 in Human fetal kidney whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Map17 was immunoprecipitated from 1mg of Human fetal kidney whole cell lysate with ab199540 at 1/300 dilution. Western blot was performed from the immunoprecipitate using anti-Map17 (ab156014) at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Human fetal kidney whole cell lysate 10ug (Input). Lane 2: ab199540 IP in Human fetal kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199540 in Human fetal kidney whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

References

ab199540 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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