Overview

  • Product name

    Anti-MAP1A antibody [EPR18993]
    See all MAP1A primary antibodies
  • Description

    Rabbit monoclonal [EPR18993] to MAP1A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse MAP1A aa 350-600. The exact sequence is proprietary.
    Database link: Q9QYR6

  • Positive control

    • IHC-P: Human cerebrum, mouse cerebellum and rat cerebellum tissues. IHC-Fr: Mouse cerebellum tissue. IP: Mouse brain whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
IHC-Fr 1/500.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements.
  • Tissue specificity

    Brain.
  • Sequence similarities

    Belongs to the MAP1 family.
  • Domain

    The basic region containing the repeats may be responsible for the binding of MAP1A to microtubules.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    LC2 is generated from MAP1A by proteolytic processing.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • MAP 1A antibody
    • MAP 1L antibody
    • MAP-1A antibody
    • MAP1 light chain LC2 antibody
    • Map1a antibody
    • MAP1A_HUMAN antibody
    • MAP1L antibody
    • Microtubule associated protein 1 like antibody
    • Microtubule associated protein 1A antibody
    • MTAP 1A antibody
    • MTAP1A antibody
    • Proliferation related protein p80 antibody
    • Proliferation-related protein p80 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling MAP1A with ab184349 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of the normal Human cerebrum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is  ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MAP1A with ab184349 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on normal Human colon. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling MAP1A with ab184349 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human breast carcinoma. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling MAP1A with ab184349 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on purkinje cells of the mouse cerebellum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MAP1A with ab184349 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse kidney. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling MAP1A with ab184349 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on purkinje cells of the rat cerebellum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MAP1A with ab184349 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat colon. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling MAP1A with ab184349 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed cytoplasm staining on purkinje cells of the mouse cerebellum. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling MAP1A with ab184349 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed negative staining on mouse kidney. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • MAP1A was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab184349 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab184349 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse brain whole cell lysate, 10µg (Input).

    Lane 2: ab184349 IP in Mouse brain whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab184349 in Mouse brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

ab184349 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (GT1-7 Mouse Hypothalamic GnRH Neuronal Cell Line)
Permeabilization
Yes - 0.1% triton X-100
Specification
GT1-7 Mouse Hypothalamic GnRH Neuronal Cell Line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 27 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up