• Product name

    Anti-MAP1B antibody [AA6]
    See all MAP1B primary antibodies
  • Description

    Mouse monoclonal [AA6] to MAP1B
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Cat, Human
  • Immunogen

    Rat brain microtubule associated proteins.

  • Positive control

    • Rat brain.
  • General notes

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.




Our Abpromise guarantee covers the use of ab11266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC/IF: Use at an assay dependent dilution in conjunction with either immunofluorescence or immunoperoxidase labelling methods.
    WB: 1/500. Predicted molecular weight: 270 kDa. May run higher due to many phosphorylation sites: 320kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Facilitates tyrosination of alpha-tubulin in neuronal microtubules (By similarity). Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.
    • Sequence similarities

      Belongs to the MAP1 family.
    • Domain

      Has a highly basic region with many copies of the sequence KKEE and KKEI/V, repeated but not at fixed intervals, which is responsible for the binding of MAP1B to microtubules.
    • Post-translational

      LC1 is generated from MAP1B by proteolytic processing.
      S-nitrosylation at Cys-2464 enhances interaction with microtubules, and may act as an effector modification for neuronal nitric oxide synthase control of growth-cone size, growth-cone collapse and axon retraction.
    • Cellular localization

      Cytoplasm, cytoskeleton. Cytoplasm. Cell junction, synapse. Cell projection, dendritic spine. Colocalizes with DAPK1 in the microtubules and cortical actin fibers.
    • Information by UniProt
    • Database links

    • Alternative names

      • FUTSCH antibody
      • LC1 antibody
      • MAP-1B antibody
      • MAP1 light chain LC1 antibody
      • Map1b antibody
      • MAP1B_HUMAN antibody
      • MAP5 antibody
      • Microtubule associated protein 1B antibody
      • Mtap1b antibody
      • Mtap5 antibody
      see all


    • ab11266 (1/300) staining MAP1B (centre column) in Human cortical neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Left column staining: tubulin, actin, synapsin, SAP102. Right column: overlay.


    This product has been referenced in:

    • Sakano H  et al. Proteomic analyses of nucleus laminaris identified candidate targets of the fragile X mental retardation protein. J Comp Neurol 525:3341-3359 (2017). Read more (PubMed: 28685837) »
    • Eriksson M  et al. MAP1B binds to the NMDA receptor subunit NR3A and affects NR3A protein concentrations. Neurosci Lett 475:33-7 (2010). WB, ICC/IF ; Rat . Read more (PubMed: 20304030) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M Citrate buffer pH6.0+Tween 0.05%
    Mouse Tissue sections (brain)
    Yes - 0.2%Triton X100

    Abcam user community

    Verified customer

    Submitted Aug 08 2014


    Thank you for contacting us for technical support. I'm sorry to hear you are experiencing problems with your recent vial of ab11266. We have not received any other complaints about this very popular antibody. Could you please provide your order number and I will look into possible shipping delays which would indicate a damage during shipping or storage? The antibody is made as ascites (i.e. unpurified antibody) therefore the concentration of antibody varies from lot to lot and you may need to vary the dilution of the antibody. If you could please tell me what dilutions you have tried and incubation times, this would help me understand your problem. I look forward to hearing from you,

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    Thank you for your enquiry. I have received the following information from our source for this antibody, which I hope will be useful. I have added the publications listed below, along with the PubMed links, to the online datasheet for ab11266. "This antibody, clone AA6, recognizes a conserved nonphosphorylated and nonphosphorylatable epitope on MAP1b. See: Paglini, G., et al., Evidence for the Participation of the Neuron-Specific CDK5 Activator P35 during Laminin-Enhanced Axonal Growth. J. Neuroscience, 18(23), 9858-9869 (1998). DiTella, M., et al., MAP1B/tau functional redundancy during laminin-enhanced axonal growth. J. Cell. Sci., 109, 467-477 (1996). It reacts with all isoforms of MAP1b, in both Western blot and immunofluorescence applications. See: Franzen, R., et al., Microtubule-associated protein 1B: a neuronal binding partner for myelin-associated glycoprotein. J. Cell Biol., 155(6), 893-898 (2001). I searched all three of the papers cited above, but I did not see any mention of heavy or light chains of MAP1B. In particular, the Franzen paper shows several Western blots with a band that has a MW of about 250 kDa, which I think is the "full size" MAP1B. I'm curious...what was the indication that MAP1B has a heavy chain and light chain?" Thank you, and have a great weekend!

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    Thank you for your enquiry. I have contacted our lab and I have discovered that given that this antibody was raised using rat brain microtubule associated proteins and unfortunately there has been no follow up to map the epitope. I also performed some searching using the EXPASY database and correct me if I am wrong but it appears that the domains of the light and heavy chains are yet to be defined. (see Key Features at: http://ca.expasy.org/uniprot/P15205). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Thank you for your enquiry and your interest in our products. This antibody has been tested and characterized using newborn rat brain. Unfortunately, we have not particularly tested in Pericytes.

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