Anti-MAP1B antibody (ab154333)
Key features and details
- Rabbit polyclonal to MAP1B
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MAP1B antibody
See all MAP1B primary antibodies -
Description
Rabbit polyclonal to MAP1B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 1824-1878 of Human MAP1B (UniProt: P46821).
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Positive control
- U-87 MG, P4.5 Mouse brain lysate, NT2D1, IMR32 and Neuro-2a whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 271 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 271 kDa. |
Target
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Function
Facilitates tyrosination of alpha-tubulin in neuronal microtubules (By similarity). Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing. -
Sequence similarities
Belongs to the MAP1 family. -
Domain
Has a highly basic region with many copies of the sequence KKEE and KKEI/V, repeated but not at fixed intervals, which is responsible for the binding of MAP1B to microtubules. -
Post-translational
modificationsLC1 is generated from MAP1B by proteolytic processing.
S-nitrosylation at Cys-2464 enhances interaction with microtubules, and may act as an effector modification for neuronal nitric oxide synthase control of growth-cone size, growth-cone collapse and axon retraction. -
Cellular localization
Cytoplasm, cytoskeleton. Cytoplasm. Cell junction, synapse. Cell projection, dendritic spine. Colocalizes with DAPK1 in the microtubules and cortical actin fibers. - Information by UniProt
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Database links
- Entrez Gene: 4131 Human
- Entrez Gene: 17755 Mouse
- Omim: 157129 Human
- SwissProt: P46821 Human
- SwissProt: P14873 Mouse
- Unigene: 335079 Human
- Unigene: 4173 Mouse
- Unigene: 474609 Mouse
see all -
Alternative names
- FUTSCH antibody
- LC1 antibody
- MAP-1B antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154333 has not yet been referenced specifically in any publications.