WB: Soluble fraction of Y79 cell extract, 293 transfected with Human MAP1LC3A and Hela cell lysates treated with bafilomycin overnight. IF: Mouse cerebellar cell lines stably expressing human GFP-MAP1LC3A fusion protein. IHC: Muscle tissue of a diseased mouse off Dox after 5 weeks on regular food.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 14 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/10 - 1/50. methanol:acetone fixation
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
Belongs to the MAP1 LC3 family.
The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
Hao Y et al. Autophagy mediates the degradation of synaptic vesicles: A potential mechanism of synaptic plasticity injury induced by microwave exposure in rats. Physiol Behav188:119-127 (2018).
Read more (PubMed: 29408588) »
Larroquette F et al. Vapb/Amyotrophic lateral sclerosis 8 knock-in mice display slowly progressive motor behavior defects accompanying ER stress and autophagic response. Hum Mol Genet24:6515-29 (2015).
Read more (PubMed: 26362257) »