Overview

  • Product name
    Anti-MAP1LC3A antibody [166AT1234]
    See all MAP1LC3A primary antibodies
  • Description
    Mouse monoclonal [166AT1234] to MAP1LC3A
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-121 of Human MAP1LC3A (UniProt ID: Q9H492).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • WB: Soluble fraction of Y79 cell extract, 293 transfected with Human MAP1LC3A and Hela cell lysates treated with bafilomycin overnight.
      IF: Mouse cerebellar cell lines stably expressing human GFP-MAP1LC3A fusion protein.
      IHC: Muscle tissue of a diseased mouse off Dox after 5 weeks on regular food.

Properties

Applications

Our Abpromise guarantee covers the use of ab168803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 14 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/10 - 1/50. methanol:acetone fixation

Target

  • Function
    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificity
    Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the MAP1 LC3 family.
  • Post-translational
    modifications
    The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
  • Cellular localization
    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ATG8E antibody
    • Autophagy-related protein LC3 A antibody
    • Autophagy-related ubiquitin-like modifier LC3 A antibody
    • LC3 antibody
    • LC3A antibody
    • MAP1 light chain 3 like protein 1 antibody
    • MAP1 light chain 3-like protein 1 antibody
    • MAP1A/1B light chain 3 A antibody
    • MAP1A/MAP1B LC3 A antibody
    • MAP1A/MAP1B light chain 3 A antibody
    • MAP1ALC3 antibody
    • MAP1BLC3 antibody
    • Map1lc3a antibody
    • Microtubule associated proteins 1A/1B light chain 3 antibody
    • Microtubule-associated protein 1 light chain 3 alpha antibody
    • Microtubule-associated proteins 1A and 1B, light chain 3 antibody
    • Microtubule-associated proteins 1A/1B light chain 3A antibody
    • MLP3A_HUMAN antibody
    see all

Images

  • Western blot - Anti-MAP1LC3A antibody [166AT1234] (ab168803)
    Western blot - Anti-MAP1LC3A antibody [166AT1234] (ab168803)
    All lanes : Anti-MAP1LC3A antibody [166AT1234] (ab168803) at 1/1000 dilution

    Lane 1 : Hela cell lysates treated with DMSO
    Lane 2 : Hela cell lysates treated with rapamycin
    Lane 3 : Hela cell lysates treated with bafilomycin


    Lysates/proteins at 12.5 µg per lane.

    Predicted band size: 14 kDa

  • Western blot - Anti-MAP1LC3A antibody [166AT1234] (ab168803)
    Western blot - Anti-MAP1LC3A antibody [166AT1234] (ab168803)
    All lanes : Anti-MAP1LC3A antibody [166AT1234] (ab168803) at 8 µg/ml

    Lane 1 : Y79 (soluble fraction of cell extract)
    Lane 2 : 293 transfected with human MAP1LC3A (whole cell extract)

    Lysates/proteins at 12.5 µg per lane.

    Predicted band size: 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [166AT1234] (ab168803)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [166AT1234] (ab168803)
    Immunohistochemistry analysis of muscle tissue of a diseased Mouse off Dox after 5 weeks on regular food CD2 labeling MAP1LC3A with ab168803 at 1/50.
  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [166AT1234] (ab168803)
    Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [166AT1234] (ab168803)
    Immunocytochemistry analysis of Mouse cerebellar cell lines stably expressing Human GFP-MAP1LC3A fusion protein labeling MAP1LC3A with ab168803 at 1/10. Conditions: 1:1 methanol:acetone fixation, 0.2% saponin permeabilization.
  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [166AT1234] (ab168803)
    Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [166AT1234] (ab168803)
    Immunocytochemistry analysis of Cathepsin D WT (bottom) and KO (top) Mouse thalamus (postnatal day 25) labeling MAP1LC3A with ab168803 at 1/10.

References

This product has been referenced in:
  • Hao Y  et al. Autophagy mediates the degradation of synaptic vesicles: A potential mechanism of synaptic plasticity injury induced by microwave exposure in rats. Physiol Behav 188:119-127 (2018). ICC/IF . Read more (PubMed: 29408588) »
  • Larroquette F  et al. Vapb/Amyotrophic lateral sclerosis 8 knock-in mice display slowly progressive motor behavior defects accompanying ER stress and autophagic response. Hum Mol Genet 24:6515-29 (2015). Read more (PubMed: 26362257) »

See all 2 Publications for this product

Publishing research using ab168803? Please let us know so that we can cite the reference in this datasheet.

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