Anti-MAP1LC3A antibody [166AT1234] (ab168803)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-MAP1LC3A antibody [166AT1234]
See all MAP1LC3A primary antibodies -
DescriptionMouse monoclonal [166AT1234] to MAP1LC3A
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Recombinant full length protein corresponding to amino acids 1-121 of Human MAP1LC3A (UniProt ID: Q9H492).
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Positive control
- WB: Soluble fraction of Y79 cell extract, 293 transfected with Human MAP1LC3A and Hela cell lysates treated with bafilomycin overnight.
IF: Mouse cerebellar cell lines stably expressing human GFP-MAP1LC3A fusion protein.
IHC: Muscle tissue of a diseased mouse off Dox after 5 weeks on regular food.
- WB: Soluble fraction of Y79 cell extract, 293 transfected with Human MAP1LC3A and Hela cell lysates treated with bafilomycin overnight.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein G purified
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Purification notesab168803 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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ClonalityMonoclonal
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Clone number166AT1234
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab168803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 14 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/10 - 1/50. methanol:acetone fixation |
Target
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FunctionProbably involved in formation of autophagosomal vacuoles (autophagosomes).
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Tissue specificityMost abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
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Sequence similaritiesBelongs to the MAP1 LC3 family.
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Post-translational
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. -
Cellular localizationCytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
- Information by UniProt
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Database links
- Entrez Gene: 84557 Human
- Entrez Gene: 66734 Mouse
- Entrez Gene: 362245 Rat
- Omim: 601242 Human
- SwissProt: Q9H492 Human
- SwissProt: Q91VR7 Mouse
- SwissProt: Q6XVN8 Rat
- Unigene: 632273 Human
see all -
Alternative names
- ATG8E antibody
- Autophagy-related protein LC3 A antibody
- Autophagy-related ubiquitin-like modifier LC3 A antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [166AT1234] (ab168803)](https://www.abcam.com/ps/products/168/ab168803/Images/MAP1LC3A-Primary-antibodies-ab168803-10.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [166AT1234] (ab168803)Immunohistochemistry analysis of muscle tissue of a diseased Mouse off Dox after 5 weeks on regular food CD2 labeling MAP1LC3A with ab168803 at 1/50. -
![Western blot - Anti-MAP1LC3A antibody [166AT1234] (ab168803)](https://www.abcam.com/ps/products/168/ab168803/Images/MAP1LC3A-Primary-antibodies-ab168803-4.jpg)
Western blot - Anti-MAP1LC3A antibody [166AT1234] (ab168803)All lanes : Anti-MAP1LC3A antibody [166AT1234] (ab168803) at 1/1000 dilution
Lane 1 : Hela cell lysates treated with DMSO
Lane 2 : Hela cell lysates treated with rapamycin
Lane 3 : Hela cell lysates treated with bafilomycin
Lysates/proteins at 12.5 µg per lane.
Predicted band size: 14 kDa -
![Western blot - Anti-MAP1LC3A antibody [166AT1234] (ab168803)](https://www.abcam.com/ps/products/168/ab168803/Images/MAP1LC3A-Primary-antibodies-ab168803-6.jpg)
Western blot - Anti-MAP1LC3A antibody [166AT1234] (ab168803)All lanes : Anti-MAP1LC3A antibody [166AT1234] (ab168803) at 8 µg/ml
Lane 1 : Y79 (soluble fraction of cell extract)
Lane 2 : 293 transfected with human MAP1LC3A (whole cell extract)
Lysates/proteins at 12.5 µg per lane.
Predicted band size: 14 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [166AT1234] (ab168803)](https://www.abcam.com/ps/products/168/ab168803/Images/MAP1LC3A-Primary-antibodies-ab168803-8.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [166AT1234] (ab168803)Immunocytochemistry analysis of Mouse cerebellar cell lines stably expressing Human GFP-MAP1LC3A fusion protein labeling MAP1LC3A with ab168803 at 1/10. Conditions: 1:1 methanol:acetone fixation, 0.2% saponin permeabilization. -
![Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [166AT1234] (ab168803)](https://www.abcam.com/ps/products/168/ab168803/Images/MAP1LC3A-Primary-antibodies-ab168803-9.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [166AT1234] (ab168803)Immunocytochemistry analysis of Cathepsin D WT (bottom) and KO (top) Mouse thalamus (postnatal day 25) labeling MAP1LC3A with ab168803 at 1/10.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Hao Y et al. Autophagy mediates the degradation of synaptic vesicles: A potential mechanism of synaptic plasticity injury induced by microwave exposure in rats. Physiol Behav 188:119-127 (2018). ICC/IF . Read more (PubMed: 29408588) »
- Larroquette F et al. Vapb/Amyotrophic lateral sclerosis 8 knock-in mice display slowly progressive motor behavior defects accompanying ER stress and autophagic response. Hum Mol Genet 24:6515-29 (2015). Read more (PubMed: 26362257) »
