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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules MT Associated Proteins MAP
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Anti-MAP1LC3A antibody [BS405] (ab244210)

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Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [BS405] (ab244210)
  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [BS405] (ab244210)
  • Flow Cytometry - Anti-MAP1LC3A antibody [BS405] (ab244210)
  • Western blot - Anti-MAP1LC3A antibody [BS405] (ab244210)

Key features and details

  • Mouse monoclonal [BS405] to MAP1LC3A
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-MAP1LC3A antibody [BS405]
    See all MAP1LC3A primary antibodies
  • Description

    Mouse monoclonal [BS405] to MAP1LC3A
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human MAP1LC3A aa 11-23. Conjugated to a carrier protein.
    Sequence:

    RSFADRCKEVQQI


    Database link: Q9H492
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Flow Cyt: SH-SY5Y cells. ICC/IF: SH-SY5Y and C6 cells. WB: SH-SY5Y cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Lyophilized:Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives. Highest purity glycerol (1:1) may be added for additional stability.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Constituents: PBS, 3% Trehalose
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    BS405
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Cardiovascular
    • Heart
    • Autophagy
    • Autophagosome
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Autophagosome
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Cancer
    • Cell Death
    • Autophagy
    • Autophagosome

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Recombinant Protein

    • Recombinant Human MAP1LC3A protein (ab98119)
  • Related Products

    • Recombinant Human MAP1LC3A protein (BSA and azide free) (ab171754)
    • Recombinant Human MAP1LC3A protein (ab98119)

Applications

Our Abpromise guarantee covers the use of ab244210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Predicted molecular weight: 14 kDa.
ICC/IF Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use a concentration of 20 µg/ml.

Target

  • Function

    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificity

    Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the MAP1 LC3 family.
  • Post-translational
    modifications

    The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
  • Cellular localization

    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Target information above from: UniProt accession Q9H492 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 84557 Human
    • Entrez Gene: 66734 Mouse
    • Entrez Gene: 362245 Rat
    • Omim: 601242 Human
    • SwissProt: Q9H492 Human
    • SwissProt: Q91VR7 Mouse
    • SwissProt: Q6XVN8 Rat
    • Unigene: 632273 Human
    • Unigene: 196239 Mouse
    • Unigene: 3135 Rat
    see all
  • Alternative names

    • ATG8E antibody
    • Autophagy related protein LC3 A antibody
    • Autophagy related ubiquitin like modifier LC3 A antibody
    • Autophagy-related protein LC3 A antibody
    • Autophagy-related ubiquitin-like modifier LC3 A antibody
    • LC3 antibody
    • LC3A antibody
    • MAP1 light chain 3 like protein 1 antibody
    • MAP1 light chain 3-like protein 1 antibody
    • MAP1A/1B light chain 3 A antibody
    • MAP1A/MAP1B LC3 A antibody
    • MAP1A/MAP1B light chain 3 A antibody
    • MAP1ALC3 antibody
    • MAP1BLC3 antibody
    • Map1lc3a antibody
    • Microtubule associated proteins 1A/1B light chain 3 antibody
    • Microtubule-associated protein 1 light chain 3 alpha antibody
    • Microtubule-associated proteins 1A and 1B, light chain 3 antibody
    • Microtubule-associated proteins 1A/1B light chain 3A antibody
    • MLP3A_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [BS405] (ab244210)
    Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [BS405] (ab244210)

    Immunofluorescence analysis of MAP1LC3A expression in SH-SY5Y (human neuroblastoma cell line from bone marrow) cells.
    Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X-100) C6 cells were incubated with ab244210 at 2 μg/mL, (green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [BS405] (ab244210)
    Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [BS405] (ab244210)

    Immunofluorescence analysis of MAP1LC3A expression in C6 (rat glial tumor cell line) cells.
    Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X-100) C6 cells were incubated with ab244210 at 2 μg/mL, (green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue).

  • Flow Cytometry - Anti-MAP1LC3A antibody [BS405] (ab244210)
    Flow Cytometry - Anti-MAP1LC3A antibody [BS405] (ab244210)

    Flow cytometry analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells of MAP1LC3A with ab244210.
    Fixing / permeabilization of cells: Absolute methanol / 0.1% Tween-20 in PBS.
    Primary antibody: ab244210 at 2 μg per ~106 cells for 15 minutes at room temperature.
    Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1/1000) with incubation for 15 minutes in dark at room temperature.
    Non-specific Control IgG, clone X63 was used under same conditions as negative control (black dashed).

  • Western blot - Anti-MAP1LC3A antibody [BS405] (ab244210)
    Western blot - Anti-MAP1LC3A antibody [BS405] (ab244210)
    Anti-MAP1LC3A antibody [BS405] (ab244210) at 10 µg/ml (overnight at 4degC) + SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 20 µg

    Secondary
    Anti-mouse-HRP, 2 hrs at RT at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 14 kDa



    SDS-PAGE: 4-20% Bis-Tris denatured.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab244210? Please let us know so that we can cite the reference in this datasheet.

    ab244210 has not yet been referenced specifically in any publications.

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