Anti-MAP1LC3A antibody [BS405] (ab244210)
Key features and details
- Mouse monoclonal [BS405] to MAP1LC3A
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-MAP1LC3A antibody [BS405]
See all MAP1LC3A primary antibodies -
Description
Mouse monoclonal [BS405] to MAP1LC3A -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide corresponding to Human MAP1LC3A aa 11-23. Conjugated to a carrier protein.
Sequence:RSFADRCKEVQQI
Database link: Q9H492 -
Positive control
- Flow Cyt: SH-SY5Y cells. ICC/IF: SH-SY5Y and C6 cells. WB: SH-SY5Y cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:Reconstitute in 100 uL of sterile water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives. Highest purity glycerol (1:1) may be added for additional stability. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Constituents: PBS, 3% Trehalose -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
BS405 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab244210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use a concentration of 10 µg/ml. Predicted molecular weight: 14 kDa. | |
ICC/IF | Use a concentration of 1 - 2 µg/ml. | |
Flow Cyt | Use a concentration of 20 µg/ml. |
Target
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Function
Probably involved in formation of autophagosomal vacuoles (autophagosomes). -
Tissue specificity
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. -
Sequence similarities
Belongs to the MAP1 LC3 family. -
Post-translational
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. -
Cellular localization
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes. - Information by UniProt
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Database links
- Entrez Gene: 84557 Human
- Entrez Gene: 66734 Mouse
- Entrez Gene: 362245 Rat
- Omim: 601242 Human
- SwissProt: Q9H492 Human
- SwissProt: Q91VR7 Mouse
- SwissProt: Q6XVN8 Rat
- Unigene: 632273 Human
see all -
Alternative names
- ATG8E antibody
- Autophagy related protein LC3 A antibody
- Autophagy related ubiquitin like modifier LC3 A antibody
see all
Images
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Immunofluorescence analysis of MAP1LC3A expression in SH-SY5Y (human neuroblastoma cell line from bone marrow) cells.
Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X-100) C6 cells were incubated with ab244210 at 2 μg/mL, (green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). -
Immunofluorescence analysis of MAP1LC3A expression in C6 (rat glial tumor cell line) cells.
Fixed (4% formaldehyde), permeabilized, and blocked (10% normal horse serum, 0.1% Triton X-100) C6 cells were incubated with ab244210 at 2 μg/mL, (green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 μg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). -
Flow cytometry analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells of MAP1LC3A with ab244210.
Fixing / permeabilization of cells: Absolute methanol / 0.1% Tween-20 in PBS.
Primary antibody: ab244210 at 2 μg per ~106 cells for 15 minutes at room temperature.
Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1/1000) with incubation for 15 minutes in dark at room temperature.
Non-specific Control IgG, clone X63 was used under same conditions as negative control (black dashed). -
Anti-MAP1LC3A antibody [BS405] (ab244210) at 10 µg/ml (overnight at 4degC) + SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 20 µg
Secondary
Anti-mouse-HRP, 2 hrs at RT at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 14 kDaSDS-PAGE: 4-20% Bis-Tris denatured.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab244210 has not yet been referenced specifically in any publications.