Recombinant Anti-MAP1LC3A antibody [EP1528Y] (ab52628)
- Datasheet
- References (7)
- Protocols
Overview
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Product name
Anti-MAP1LC3A antibody [EP1528Y]
See all MAP1LC3A primary antibodies -
Description
Rabbit monoclonal [EP1528Y] to MAP1LC3A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human MAP1LC3A aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9H492 -
Positive control
- Fetal brain cytoplasmic cell lysate Human brain tissue ICC/IF: SH-SY5Y cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1528Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab52628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | 1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa). | |
| IP | 1/100. | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Probably involved in formation of autophagosomal vacuoles (autophagosomes). -
Tissue specificity
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. -
Sequence similarities
Belongs to the MAP1 LC3 family. -
Post-translational
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. -
Cellular localization
Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes. - Information by UniProt
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Database links
- Entrez Gene: 84557 Human
- Entrez Gene: 66734 Mouse
- Entrez Gene: 362245 Rat
- Omim: 601242 Human
- SwissProt: Q9H492 Human
- SwissProt: Q91VR7 Mouse
- SwissProt: Q6XVN8 Rat
- Unigene: 632273 Human
see all -
Alternative names
- ATG8E antibody
- Autophagy related protein LC3 A antibody
- Autophagy related ubiquitin like modifier LC3 A antibody
see all
Images
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![Western blot - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)](https://www.abcam.com/ps/products/52/ab52628/Images/ab52628-64999-anti-map1lc3a-antibody-ep1528y-western-blot.jpg)
Western blot - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)Anti-MAP1LC3A antibody [EP1528Y] (ab52628) at 1/50000 dilution + Fetal brain lysate at 10 µg
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)](https://www.abcam.com/ps/products/52/ab52628/Images/ab52628-4-anti-map1lc3a-antibody-ep1528y-immunocytochemistry-shsy5y-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (human neuroblastoma epithelial cell) cells labeling MAP1LC3A with purified ab52628 at 1/100 dilution (10 µg/mL). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)](https://www.abcam.com/ps/products/52/ab52628/Images/ab52628-64997-anti-map1lc3a-antibody-ep1528y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)IHC image of ab52628 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52628, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References
This product has been referenced in:
- Vishnyakova PA et al. Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium. BBA Clin 8:35-42 (2017). Read more (PubMed: 28736722) »
- Cabang AB et al. Therapeutic effects of the euglenoid ichthyotoxin, euglenophycin, in colon cancer. Oncotarget 8:104347-104358 (2017). Read more (PubMed: 29262645) »
