Recombinant
RabMAb

Anti-MAP1LC3A antibody [EP1528Y] (ab52628)

Abreviews (8) Submit a Question Specific References (7)

Overview

  • Product name
    Anti-MAP1LC3A antibody [EP1528Y]
    See all MAP1LC3A primary antibodies
  • Description
    Rabbit monoclonal [EP1528Y] to MAP1LC3A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MAP1LC3A (N terminal). The exact sequence is proprietary.

  • Positive control
    • Fetal brain cytoplasmic cell lysate Human brain tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab52628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
IP 1/100.
ICC 1/50 - 1/100.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function
      Probably involved in formation of autophagosomal vacuoles (autophagosomes).
    • Tissue specificity
      Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
    • Sequence similarities
      Belongs to the MAP1 LC3 family.
    • Post-translational
      modifications
      The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
    • Cellular localization
      Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • ATG8E antibody
      • Autophagy-related protein LC3 A antibody
      • Autophagy-related ubiquitin-like modifier LC3 A antibody
      • LC3 antibody
      • LC3A antibody
      • MAP1 light chain 3 like protein 1 antibody
      • MAP1 light chain 3-like protein 1 antibody
      • MAP1A/1B light chain 3 A antibody
      • MAP1A/MAP1B LC3 A antibody
      • MAP1A/MAP1B light chain 3 A antibody
      • MAP1ALC3 antibody
      • MAP1BLC3 antibody
      • Map1lc3a antibody
      • Microtubule associated proteins 1A/1B light chain 3 antibody
      • Microtubule-associated protein 1 light chain 3 alpha antibody
      • Microtubule-associated proteins 1A and 1B, light chain 3 antibody
      • Microtubule-associated proteins 1A/1B light chain 3A antibody
      • MLP3A_HUMAN antibody
      see all

    Images

    • Western blot - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)
      Western blot - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)
      Anti-MAP1LC3A antibody [EP1528Y] (ab52628) at 1/50000 dilution + Fetal brain lysate at 10 µg

      Secondary
      goat anti-rabbit HRP labelled at 1/2000 dilution

      Predicted band size: 14 kDa
      Observed band size: 16 kDa (why is the actual band size different from the predicted?)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)
      IHC image of ab52628 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52628, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References

    This product has been referenced in:
    • Vishnyakova PA  et al. Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium. BBA Clin 8:35-42 (2017). Read more (PubMed: 28736722) »
    • Cabang AB  et al. Therapeutic effects of the euglenoid ichthyotoxin, euglenophycin, in colon cancer. Oncotarget 8:104347-104358 (2017). Read more (PubMed: 29262645) »

    See all 7 Publications for this product

    Publishing research using ab52628? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    8 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-MAP1LC3A antibody [EP1528Y]

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (nerublastoma)
    Specification
    nerublastoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 16°C
    Read More
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    Abcam user community

    Verified customer

    Submitted Apr 28 2014

    Western blot abreview for Anti-MAP1LC3A antibody [EP1528Y]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Liver)
    Loading amount
    30 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Abcam user community

    Verified customer

    Submitted Jun 25 2012

    Western blot abreview for Anti-MAP1LC3A antibody [EP1528Y]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Loading amount
    30 µg
    Specification
    Liver
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Abcam user community

    Verified customer

    Submitted Jun 25 2012

    Western blot abreview for Anti-MAP1LC3A antibody [EP1528Y]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Lung)
    Loading amount
    50 µg
    Specification
    Lung
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Abcam user community

    Verified customer

    Submitted Apr 02 2012

    Western blot abreview for Anti-MAP1LC3A antibody [EP1528Y]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (kidney)
    Loading amount
    30 µg
    Specification
    kidney
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Verified customer

    Submitted Jun 13 2011

    Western blot abreview for Anti-MAP1LC3A antibody [EP1528Y]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (liver)
    Loading amount
    50 µg
    Specification
    liver
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Abcam user community

    Verified customer

    Submitted Jun 08 2011

    Western blot abreview for Anti-MAP1LC3A antibody [EP1528Y]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver)
    Loading amount
    15 µg
    Specification
    liver
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Abcam user community

    Verified customer

    Submitted Jan 05 2011

    Western blot abreview for Anti-MAP1LC3A antibody [EP1528Y]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver, heart, brain, lung, muscle, small bowel, sp)
    Loading amount
    20 µg
    Specification
    liver, heart, brain, lung, muscle, small bowel, sp
    Gel Running Conditions
    Non-reduced Denaturing (15)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Jun 05 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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