Recombinant
Recombinant Anti-MAP1LC3A antibody [EP1528Y] (ab52628)
- Datasheet
- References (7)
- Protocols
Overview
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Product nameAnti-MAP1LC3A antibody [EP1528Y]
See all MAP1LC3A primary antibodies -
DescriptionRabbit monoclonal [EP1528Y] to MAP1LC3A
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, ICC, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human MAP1LC3A aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9H492 -
Positive control
- Fetal brain cytoplasmic cell lysate Human brain tissue
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP1528Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab52628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/50000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa). | |
| IP | 1/100. | |
| ICC | 1/50 - 1/100. | |
| IHC-P | Use at an assay dependent concentration. |
Application notes
Is unsuitable for Flow Cyt.
Target
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FunctionProbably involved in formation of autophagosomal vacuoles (autophagosomes).
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Tissue specificityMost abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
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Sequence similaritiesBelongs to the MAP1 LC3 family.
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Post-translational
modificationsThe precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. -
Cellular localizationCytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
- Information by UniProt
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Database links
- Entrez Gene: 84557 Human
- Entrez Gene: 66734 Mouse
- Entrez Gene: 362245 Rat
- Omim: 601242 Human
- SwissProt: Q9H492 Human
- SwissProt: Q91VR7 Mouse
- SwissProt: Q6XVN8 Rat
- Unigene: 632273 Human
see all -
Alternative names
- ATG8E antibody
- Autophagy-related protein LC3 A antibody
- Autophagy-related ubiquitin-like modifier LC3 A antibody
see all
Images
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![Western blot - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)](https://www.abcam.com/ps/products/52/ab52628/Images/ab52628-64999-anti-map1lc3a-antibody-ep1528y-western-blot.jpg)
Western blot - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)Anti-MAP1LC3A antibody [EP1528Y] (ab52628) at 1/50000 dilution + Fetal brain lysate at 10 µg
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)](https://www.abcam.com/ps/products/52/ab52628/Images/ab52628-64997-anti-map1lc3a-antibody-ep1528y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP1LC3A antibody [EP1528Y] (ab52628)IHC image of ab52628 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52628, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
References
This product has been referenced in:
- Vishnyakova PA et al. Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium. BBA Clin 8:35-42 (2017). Read more (PubMed: 28736722) »
- Cabang AB et al. Therapeutic effects of the euglenoid ichthyotoxin, euglenophycin, in colon cancer. Oncotarget 8:104347-104358 (2017). Read more (PubMed: 29262645) »
