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Synthetic peptide within Human MAP1LC3A aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q9H492
Our Abpromise guarantee covers the use of ab173700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 14 kDa.|
|ICC/IF||1/10 - 1/50.|
lipidated MAP1LC3A (arrow, II) and non-lipidated MAP1LC3A (arrow, I)
Immunofluorescent analysis of U251 cells labeling MAP1LC3A with ab173700. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ab173700 (1/200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).
Immunofluorescent analysis of Wild-type and mutant cerebellar cells labeling MAP1LC3A with ab173700 at 1/10 dilution (Red). Co-immunostaining of CLN3-encoded protein, battenin with a monoclonal anti-battenin antibody (Green). DAPI staining (Blue).
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling MAP1LC3A with ab173700 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
ab173700 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"