Overview

  • Product name
  • Description
    Rabbit polyclonal to MAP1S
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MAP1S aa 500-528 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q66K74

  • Positive control
    • CEM cell line lysate; Human colon carcinoma tissue; CEM cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab175503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 112 kDa.

Target

  • Function
    Microtubule-associated protein that mediates aggregation of mitochondria resulting in cell death and genomic destruction (MAGD). Plays a role in anchoring the microtubule-organizing center to the centrosomes. Binds to DNA. Plays a role in apoptosis. Involved in the formation of microtubule bundles.
  • Tissue specificity
    Expressed in neurons (at protein level). Expressed in spermatocytes, spermatids and spermatozoa. Expressed in the cerebral cortex. Highly expressed in testis. Moderately expressed in the brain, colon, heart, kidney, liver, lung, placenta, small intestine, spleen and stomach. Weakly expressed in muscle.
  • Sequence similarities
    Belongs to the MAP1 family.
  • Domain
    The N-terminus of the heavy chain associates with the C-terminus of the light chain to form the heterodimer complex (By similarity). Its C-terminal part of the heavy chain interacts with ESR1.
  • Cellular localization
    Nucleus. Cytoplasm > cytosol. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > spindle. Detected in filopodia-like protrusions and synapses (By similarity). Detected in perinuclear punctate network corresponding to mitochondrial aggregates and in the nucleus in cells exhibiting apoptosis. Associated specifically with microtubules stabilized by paclitaxel and colocalizes with RASSF1 isoform A. In interphase cells, shows a diffuse cytoplasmic staining with partial localization to the microtubules. During the different stages of mitosis detected at the spindle microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • BPY2-interacting protein 1 antibody
    • BPY2IP1 antibody
    • C19orf5 antibody
    • FLJ10669 antibody
    • MAP-1S antibody
    • MAP1S antibody
    • MAP1S light chain antibody
    • MAP1S_HUMAN antibody
    • MAP8 antibody
    • MGC133087 antibody
    • microtubule-associated protein 1S antibody
    • Microtubule-associated protein 8 antibody
    • Variable charge Y chromosome 2-interacting protein 1 antibody
    • VCY2-interacting protein 1 antibody
    • VCY2IP-1 antibody
    • VCY2IP1 antibody
    see all

Images

  • Anti-MAP1S antibody (ab175503) at 1/1000 dilution + CEM cell line lysate at 35 µg

    Predicted band size: 112 kDa

  • Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling MAP1S with ab175503 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of CEM cells labeling MAP1S with ab175503 at 1/10 dilution (right histogram), compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175503 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175503.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up