Recombinant Anti-MAP2 antibody [AA6] (ab254144)

Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling MAP2 with ab254144 at 1/10000 (0.111 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human astrocytoma. The section was incubated with ab254144 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling MAP2 with ab254144 at 1/50 (22.18 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Negative control 1: ab254144 at a 1/50 dilution followed by ab150120 at a 1/500 dilution.

Negative control 2: ab11267 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling MAP2 with ab254144 at 1/10000 (0.111 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab254144 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling MAP2 with ab254144 at 1/10000 (0.111 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat cerebrum. The section was incubated with ab254144 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling MAP2 with ab254144 at 1/50 (22.18 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Negative control 1: ab254144 at a 1/50 dilution followed by ab150120 at a 1/500 dilution.

Negative control 2: ab11267 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.