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  1. Link

    map2-antibody-aa6-ab254144.pdf

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Recombinant

Recombinant Anti-MAP2 antibody [AA6] (ab254144)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] (ab254144)
  • Immunocytochemistry - Anti-MAP2 antibody [AA6] (ab254144)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] (ab254144)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] (ab254144)
  • Immunocytochemistry - Anti-MAP2 antibody [AA6] (ab254144)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AA6] to MAP2
  • Suitable for: IHC-P, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MAP2 antibody [AA6]
    See all MAP2 primary antibodies
  • Description

    Mouse monoclonal [AA6] to MAP2
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICCmore details
    Unsuitable for: Flow Cyt,IHC-Fr or WB
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Other Immunogen Type. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Human astrocytoma. Mouse and rat cerebrum tissue. ICC: Rat and mouse primary neurons.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    AA6
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Dendrite marker
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage

Associated products

  • Alternative Versions

    • Anti-MAP2 antibody [AA6] - BSA and Azide free (ab255896)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879)
  • Related Products

    • Anti-MAP2 antibody [HM-2] (ab11267)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)

Applications

Our Abpromise guarantee covers the use of ab254144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10000.
ICC 1/50.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-Fr or WB.
  • Target

    • Function

      The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
    • Sequence similarities

      Contains 3 Tau/MAP repeats.
    • Post-translational
      modifications

      Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
    • Cellular localization

      Cytoplasm, cytoskeleton.
    • Target information above from: UniProt accession P11137 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4133 Human
      • Entrez Gene: 17756 Mouse
      • Entrez Gene: 25595 Rat
      • Omim: 157130 Human
      • SwissProt: P11137 Human
      • SwissProt: P20357 Mouse
      • SwissProt: P15146 Rat
      • Unigene: 368281 Human
      • Unigene: 256966 Mouse
      • Unigene: 436793 Mouse
      • Unigene: 10484 Rat
      • Unigene: 136301 Rat
      • Unigene: 230443 Rat
      see all
    • Alternative names

      • DKFZp686I2148 antibody
      • MAP 2 antibody
      • MAP dendrite specific antibody
      • MAP-2 antibody
      • MAP2 antibody
      • MAP2A antibody
      • MAP2B antibody
      • MAP2C antibody
      • Microtubule associated protein 2 antibody
      • Microtubule-associated protein 2 antibody
      • MTAP2_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] (ab254144)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] (ab254144)

      Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling MAP2 with ab254144 at 1/10000 (0.111 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human astrocytoma. The section was incubated with ab254144 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunocytochemistry - Anti-MAP2 antibody [AA6] (ab254144)
      Immunocytochemistry - Anti-MAP2 antibody [AA6] (ab254144)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling MAP2 with ab254144 at 1/50 (22.18 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      Negative control 1: ab254144 at a 1/50 dilution followed by ab150120 at a 1/500 dilution.

      Negative control 2: ab11267 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] (ab254144)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] (ab254144)

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling MAP2 with ab254144 at 1/10000 (0.111 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab254144 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] (ab254144)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [AA6] (ab254144)

      Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling MAP2 with ab254144 at 1/10000 (0.111 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat cerebrum. The section was incubated with ab254144 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    • Immunocytochemistry - Anti-MAP2 antibody [AA6] (ab254144)
      Immunocytochemistry - Anti-MAP2 antibody [AA6] (ab254144)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling MAP2 with ab254144 at 1/50 (22.18 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron cells. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      Negative control 1: ab254144 at a 1/50 dilution followed by ab150120 at a 1/500 dilution.

      Negative control 2: ab11267 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab254144? Please let us know so that we can cite the reference in this datasheet.

    ab254144 has not yet been referenced specifically in any publications.

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