Overview

  • Product name

  • Description

    Rabbit polyclonal to MAP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, IHC-FoFr, IHC (PFA fixed), ICC, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Goat, Cat, Human
  • Immunogen

    Synthetic peptide within Rat MAP2 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P15146
    (Peptide available as ab32453)

  • Positive control

    • IHC-P: Brain (Mouse) Whole Cell Lysate - normal tissue, 0 days old, Brain (Rat) Whole Cell Lysate - normal tissue. ICC/IF: Rat PC12 cells. IHC-P: Human cerebral cortex FFPE sections.
  • General notes

      

Properties

Applications

Our Abpromise guarantee covers the use of ab32454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/200 - 1/400.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19540881
IHC (PFA fixed) Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa.Can be blocked with Rat MAP2 peptide (ab32453).

Target

  • Function

    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities

    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications

    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Images

  • Astrocytes (DIV 22) cells were fixed with 4% paraformaldehyde and stained for MAP2 (Green) using ab32454 at 1/1000 dilution in ICC/IF analysis.

  • IHC-P image of ab32454 stained lizard spinal cord. Tissue was fixed in formaldehyde with heat mediated antigen retrieval using citric acid. Tissue was blocked for 10 minutes in 1% B.S.A, and incubated with the antibody (ab32454, 1/250) for 2 hours at 21°C. The secondary antibody used was a goat-anti rabbit IgG conjugated to bitoin (1/250).

    See Abreview

  • Immunohistochemical analysis of paraformaldehyde-fixed, Triton-X100 permeabilized frozen rat spinal cord tissue labeling MAP2 (Green) using ab32454 at 1/200 dilution, followed by a Alexa Fluor® 488-conjugated goat anti-rabbit antibody at 1/500 dilution.

    Blocking step: Serum as blocking agent for 30 minute(s). Concentration: 5%. Temperature: 26°C

  • All lanes : Anti-MAP2 antibody (ab32454) at 1/1000 dilution

    Lanes 1-2 : Trisomic induced Pluripotent Stem Cells (iPSCs)
    Lanes 3-4 : Disomic induced Pluripotent Stem Cells (iPSCs)

    Predicted band size: 70, 199, 280 kDa

  • ab32454 staining MAP2 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/3000 in blocking buffer) for 2 hours at 21°C in TBS/BSA/azide. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Flow cytometric analysis of paraformaldhyde-fixed, 0.25% Triton X-100 permeabilized in DPBS Differentiated Human Neuronal stem cells stained for MAP2 using ab32454 at 1/300 dilution followed by a Alexa Fluor® 488 Goat Anti-rabbit IgG secondary antibody at 1/500 dilution. Gated on undifferentiated Stem Cells (White).

    Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS

  • ICC/IF image of ab32454 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab32454, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • ab32454 staining MAP2 in rat cryopreserved embryonic cortical neurons cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde with picric acid. Samples were incubated with primary antibody (1/2000 in 10mM PBS + 0.3% Triton-X) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • IHC image of MAP2 staining in human cerebral cortex FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32454, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References

This product has been referenced in:

  • Huang L  et al. Irisin regulates the expression of BDNF and glycometabolism in diabetic rats. Mol Med Rep 19:1074-1082 (2019). Read more (PubMed: 30569121) »
  • Liu L  et al. Icariin and icaritin ameliorated hippocampus neuroinflammation via inhibiting HMGB1-related pro-inflammatory signals in lipopolysaccharide-induced inflammation model in C57BL/6?J mice. Int Immunopharmacol 68:95-105 (2019). Read more (PubMed: 30616172) »
See all 98 Publications for this product

Customer reviews and Q&As

1-10 of 35 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.2% Triton X
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Mr. J. Y. Seo

Verified customer

Submitted Apr 04 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Induced neurons from bone marrow derived mesenchym)
Permeabilization
Yes - Triton-X100
Specification
Induced neurons from bone marrow derived mesenchym
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Paraformaldehyde

清隆 新井

Verified customer

Submitted Sep 19 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - Triton-X100
Specification
Spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Paraformaldehyde

清隆 新井

Verified customer

Submitted Sep 14 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (PC12)
Permeabilization
Yes - Triton-X100
Specification
PC12
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 14 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (induced neuron derived from pluripotent cells)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
induced neuron derived from pluripotent cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 16 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (neuron)
Permeabilization
Yes - 0.2%Triton
Specification
neuron
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 12 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain cell culture)
Specification
Brain cell culture
Blocking step
BSA as blocking agent for 13 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 12 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Cerebrum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Specification
Cerebrum
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Formaldehyde

清隆 新井

Verified customer

Submitted Sep 30 2015

Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 09 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Brain
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 18 2015

1-10 of 35 Abreviews or Q&A

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