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    map2-antibody-ab5392.pdf

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Neuroscience Cell Type Marker Neuron marker Soma marker
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Anti-MAP2 antibody (ab5392)

  • Datasheet
  • SDS
Reviews (57)Q&A (14)References (421)

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Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Western blot - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Western blot - Anti-MAP2 antibody (ab5392)

Key features and details

  • Chicken polyclonal to MAP2
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgY

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Protein
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Overview

  • Product name

    Anti-MAP2 antibody
    See all MAP2 primary antibodies
  • Description

    Chicken polyclonal to MAP2
  • Host species

    Chicken
  • Tested Applications & Species

    Application Species
    ICC
    Rat
    WB
    Rat
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human MAP2. Mix of recombinant human constructs of projection domain sequences, amino acids 235-1588.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.032% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purification notes

    Lipid extraction from egg yolk, followed by ammonium sulphate precipitation.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Dendrite marker
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Signal Transduction
    • Antibodies
    • map2

Associated products

  • Compatible Secondaries

    • Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
    • Goat Anti-Chicken IgY H&L (HRP) (ab6877)
  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Recombinant Human MAP2 protein (ab114686)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    • Anti-Alpha-synuclein antibody [LB 509] (ab27766)
    • Rat MAP2 peptide (ab32453)
    • Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Rat
WB
Rat
Application Abreviews Notes
ICC (4)
1/5000 - 1/10000.
WB (5)
1/20000 - 1/100000.

This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.

Notes
ICC
1/5000 - 1/10000.
WB
1/20000 - 1/100000.

This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.

Target

  • Function

    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities

    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications

    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Target information above from: UniProt accession P11137 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 17756 Mouse
    • Entrez Gene: 25595 Rat
    • SwissProt: P20357 Mouse
    • SwissProt: P15146 Rat
    • Unigene: 256966 Mouse
    • Unigene: 436793 Mouse
    • Unigene: 10484 Rat
    • Unigene: 136301 Rat
    • Unigene: 230443 Rat
    see all
  • Alternative names

    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Images

  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)

    Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/10000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.

  • Western blot - Anti-MAP2 antibody (ab5392)
    Western blot - Anti-MAP2 antibody (ab5392)
    All lanes : Anti-MAP2 antibody (ab5392) at 1/50000 dilution

    Lane 1 : Adult rat brain lysate
    Lane 2 : Embryonic E20 rat brain lysate
    Lane 3 : Adult mouse brain lysate
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi.
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue).
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreview
    ab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-MAP2 antibody (ab5392)
    Western blot - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreview
    Anti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg

    Secondary
    HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 280,70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

Protocols

  • Recommended IF (ICC) protocol with ab5392

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (421)

    Publishing research using ab5392? Please let us know so that we can cite the reference in this datasheet.

    ab5392 has been referenced in 421 publications.

    • Roy S  et al. Neurogenic tissue nanotransfection in the management of cutaneous diabetic polyneuropathy. Nanomedicine 28:102220 (2020). PubMed: 32422219
    • Cho Y  et al. Elevated O-GlcNAcylation induces an antidepressant-like phenotype and decreased inhibitory transmission in medial prefrontal cortex. Sci Rep 10:6924 (2020). PubMed: 32332789
    • Sharma Y  et al. In vitro human stem cell derived cultures to monitor calcium signaling in neuronal development and function. Wellcome Open Res 5:16 (2020). PubMed: 32195361
    • Pineda-Ramírez N  et al. Antioxidant Properties and Protective Effects of Some Species of the Annonaceae, Lamiaceae, and Geraniaceae Families against Neuronal Damage Induced by Excitotoxicity and Cerebral Ischemia. Antioxidants (Basel) 9:N/A (2020). PubMed: 32244955
    • Shimojo M  et al. Selective Disruption of Inhibitory Synapses Leading to Neuronal Hyperexcitability at an Early Stage of Tau Pathogenesis in a Mouse Model. J Neurosci 40:3491-3501 (2020). PubMed: 32265258
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 71 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-MAP2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Antigen retrieval step
    None
    Sample
    Rat Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Permeabilization
    Yes - 50% Ethanol 30 min
    Fixative
    Paraformaldehyde
    Read More

    Mr. Tom Owen

    Verified customer

    Submitted Apr 08 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (brain)
    Permeabilization
    Yes - 1% TritonX in PBS
    Specification
    brain
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 23 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Brain- hippocampal)
    Permeabilization
    Yes - 1% TritonX in PBS
    Specification
    Brain- hippocampal
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 23 2021

    Immunocytochemistry abreview for Anti-MAP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (iPSC-NSC)
    Permeabilization
    Yes - 0.5% triton
    Specification
    iPSC-NSC
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 16 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (iPSCs derived human neurons)
    Permeabilization
    Yes - 0.5% Triton X100
    Specification
    iPSCs derived human neurons
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Nov 30 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat primary cortical neurons)
    Permeabilization
    Yes - 0.5% Triton X100
    Specification
    Rat primary cortical neurons
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Nov 17 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse cortical culture)
    Permeabilization
    Yes - 0.2% TritonX-100 in PBS for 3hours along with blocking solution.
    Specification
    mouse cortical culture
    Blocking step
    Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Lenin Veeraval

    Verified customer

    Submitted Nov 11 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (184A1 mammary epithelial)
    Permeabilization
    Yes - PBS + 0.25% Triton-X
    Specification
    184A1 mammary epithelial
    Blocking step
    (agent) for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 19 2020

    Western blot abreview for Anti-MAP2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Meriones unguiculatus Cell lysate - whole cell (Brain (Meriones unguiculatus) Mongolian gerbil)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    25 µg
    Specification
    Brain (Meriones unguiculatus) Mongolian gerbil
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Jul 13 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Brain cell culture)
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Brain cell culture
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 21 2020

    1-10 of 71 Abreviews or Q&A

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