Anti-MAP2 antibody (ab5392)
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- References (355)
- Protocols
Overview
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Product name
Anti-MAP2 antibody
See all MAP2 primary antibodies -
Description
Chicken polyclonal to MAP2 -
Host species
Chicken -
Tested applications
Suitable for: ELISA, IHC-Fr, IHC-FoFr, IHC-P, WB, ICC/IF, IHC (PFA fixed)more details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Cow, Dog, Human, Cynomolgus monkey, Common marmoset, Aplysia -
Immunogen
Recombinant fragment corresponding to Human MAP2. Mix of recombinant human constructs of projection domain sequences, amino acids 235-1588.
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Positive control
- ICC/IF: Cultured rat cortical neurons. WB: Adult rat brain and rat embryonic E20 brain lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.032% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purification notes
Lipid extraction from egg yolk, followed by ammonium sulphate precipitation. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab5392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/5000. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-FoFr | 1/500. PubMed: 18684835 | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/20000 - 1/100000. This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands. |
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| ICC/IF | 1/5000 - 1/100000. | |
| IHC (PFA fixed) | Use at an assay dependent concentration. |
Target
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Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
Sequence similarities
Contains 3 Tau/MAP repeats. -
Post-translational
modificationsPhosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 4133 Human
- Entrez Gene: 17756 Mouse
- Entrez Gene: 25595 Rat
- Omim: 157130 Human
- SwissProt: P11137 Human
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 368281 Human
see all -
Alternative names
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody (ab5392)Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/2000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.
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Western blot - Anti-MAP2 antibody (ab5392)All lanes : Anti-MAP2 antibody (ab5392) at 1/50000 dilution
Lane 1 : Adult rat brain lysate
Lane 2 : Embryonic E20 rat brain lysate
Lane 3 : Adult mouse brain lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab5392)Image courtesy of an AbReview submitted by Mr. Carl HobbsPFA-fixed, paraffin embedded sections of sheep cerebellum were stained for MAP2 with ab5392 at 1/2000 dilution in immunohistochemical analysis. Goat Anti-Chicken IgY H&L (Biotin) (ab6876) was used as secondary antibody at 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab5392)Image courtesy of an anonymous Abreview.ab5392 staining MAP2 in murine brain tissue sections by Immunohistochemistry (PFA fixed).
Boiling in citrate-buffer was used as antigen retrieval method. ab5392 used at a 1/2000 dilution for 12 hours. The secondary used was an Alexa-Fluor 555 conjugated goat anti-chicken polyclonal used at a 1/400 dilution. -
ELISA - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreviewab5392 detecting MAP2 recombinant protein by direct ELISA. Mouse recombinat protien was coated on to microplate in carbonate coating buffer pH 9.6 for 1 hour at 37°C, Plate were blocked with 3% BSA for 1 hour at 37°C and incubated with the primary antibody (1/5000 in PBS + 1% Tween-20) for 1 hour at 37°C. An alkaline phosphatase Goat anti-chicken IgG polyclonal (1/100000) was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab5392)Image courtesy of an AbReview submitted by Mr. Carl HobbsPFA-fixed, paraffin embedded sections of rat brain were stained for MAP2 with ab5392 at 1/4000 dilution in immunohistochemical analysis. Goat Anti-Chicken IgY H&L (Biotin) (ab6876) was used as secondary antibody at 1/200 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody (ab5392)ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi. -
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody (ab5392)Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue). -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-MAP2 antibody (ab5392)This image is courtesy of an Abreview submitted by Tom Owenab5392 staining MAP2 in rat spinal cord tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and permeablized with 50% Ethanol for 30 minutes. The sample was incubated with primary antibody (1/250 in PBS + 0.3% Triton X-100) at 4°C for 48 hours. An Alexa Fluor® 488-conjugated goat anti-chicken IgY polyclonal (1/1000) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab5392)Image courtesy of an AbReview submitted by Mr. Carl HobbsPFA-fixed, paraffin embedded sections of dog brain were stained for MAP2 with ab5392 at 1/2000 dilution in immunohistochemical analysis. Goat Anti-Chicken IgY H&L (Biotin) (ab6876) was used as secondary antibody at 1/200 dilution.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreviewab5392 at 1/5000 staining PFA fixed free floating sections of mouse brain. An Alexa Fluor® 633 conjugated goat anti-chicken antibody was used as the secondary.
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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreviewab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody. -
Western blot - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous AbreviewAnti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg
Secondary
HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 280,70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
References
This product has been referenced in:
- Schafer ST et al. Pathological priming causes developmental gene network heterochronicity in autistic subject-derived neurons. Nat Neurosci N/A:N/A (2019). Read more (PubMed: 30617258) »
- Cui J et al. Metabolomics analysis explores the rescue to neurobehavioral disorder induced by maternal PM2.5 exposure in mice. Ecotoxicol Environ Saf 169:687-695 (2019). Read more (PubMed: 30500738) »
