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    map2-antibody-ab5392.pdf

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Anti-MAP2 antibody (ab5392)

  • Datasheet
  • SDS
Reviews (62)Q&A (14)References (548)

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Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Western blot - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
  • Western blot - Anti-MAP2 antibody (ab5392)

Key features and details

  • Chicken polyclonal to MAP2
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgY

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-MAP2 antibody [RM1010] (ab281588)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-MAP2 antibody
    See all MAP2 primary antibodies
  • Description

    Chicken polyclonal to MAP2
  • Host species

    Chicken
  • Tested applications

    Suitable for: ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human MAP2. Mix of recombinant human constructs of projection domain sequences, amino acids 235-1588.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.032% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purification notes

    Lipid extraction from egg yolk, followed by ammonium sulphate precipitation.
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Dendrite marker
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Signal Transduction
    • Antibodies
    • map2

Associated products

  • Compatible Secondaries

    • Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
    • Goat Anti-Chicken IgY H&L (HRP) (ab6877)
  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Recombinant Human MAP2 protein (ab114686)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-PSD95 antibody [6G6-1C9] - Synaptic Marker (ab2723)
    • Anti-Alpha-synuclein antibody [LB 509] (ab27766)
    • Rat MAP2 peptide (ab32453)
    • Anti-beta III Tubulin antibody [2G10] - Neuronal Marker (ab78078)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC (4)
1/5000 - 1/10000.
WB (5)
1/20000 - 1/100000.

This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.

Notes
ICC
1/5000 - 1/10000.
WB
1/20000 - 1/100000.

This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.

Target

  • Function

    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities

    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications

    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Target information above from: UniProt accession P11137 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 17756 Mouse
    • Entrez Gene: 25595 Rat
    • SwissProt: P20357 Mouse
    • SwissProt: P15146 Rat
    • Unigene: 256966 Mouse
    • Unigene: 436793 Mouse
    • Unigene: 10484 Rat
    • Unigene: 136301 Rat
    • Unigene: 230443 Rat
    see all
  • Alternative names

    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Images

  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)

    Rat E20 cultured cortical neuron-glial cells stained for MAP2 (red) using ab5392 at 1/10000 dilution for ICC/IF. Tau is detected with a mouse monoclonal anti-Tau antibody (green). The nuclear counter stain is DAPI (blue). Overlap of MAP2 and Tau staining results in an orange-yellow color.

  • Western blot - Anti-MAP2 antibody (ab5392)
    Western blot - Anti-MAP2 antibody (ab5392)
    All lanes : Anti-MAP2 antibody (ab5392) at 1/50000 dilution

    Lane 1 : Adult rat brain lysate
    Lane 2 : Embryonic E20 rat brain lysate
    Lane 3 : Adult mouse brain lysate
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi.
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue).
  • Immunocytochemistry - Anti-MAP2 antibody (ab5392)
    Immunocytochemistry - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreview
    ab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-MAP2 antibody (ab5392)
    Western blot - Anti-MAP2 antibody (ab5392)This image is courtesy of an anonymous Abreview
    Anti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg

    Secondary
    HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 280,70 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

Protocols

  • Recommended IF (ICC) protocol with ab5392

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (548)

Publishing research using ab5392? Please let us know so that we can cite the reference in this datasheet.

ab5392 has been referenced in 548 publications.

  • Ahn LY  et al. An epilepsy-associated ACTL6B variant captures neuronal hyperexcitability in a human induced pluripotent stem cell model. J Neurosci Res 99:110-123 (2021). PubMed: 33141462
  • McNutt PM  et al. Neuronal delivery of antibodies has therapeutic effects in animal models of botulism. Sci Transl Med 13:N/A (2021). PubMed: 33408188
  • Xu J  et al. MicroRNA expression profiling after recurrent febrile seizures in rat and emerging role of miR-148a-3p/SYNJ1 axis. Sci Rep 11:1262 (2021). PubMed: 33441699
  • Nilsson F  et al. Single-Cell Profiling of Coding and Noncoding Genes in Human Dopamine Neuron Differentiation. Cells 10:N/A (2021). PubMed: 33445654
  • Wang C  et al. ApoE-Isoform-Dependent SARS-CoV-2 Neurotropism and Cellular Response. Cell Stem Cell 28:331-342.e5 (2021). PubMed: 33450186
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 76 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
None
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Permeabilization
Yes - 50% Ethanol 30 min
Fixative
Paraformaldehyde
Read More

Mr. Tom Owen

Verified customer

Submitted Apr 08 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Striatum and Hippocampus)
Permeabilization
No
Specification
Striatum and Hippocampus
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted May 13 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse neuronal culture)
Permeabilization
No
Specification
mouse neuronal culture
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
Fixative
Methanol
Read More

Abcam user community

Verified customer

Submitted Jan 17 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cortex)
Permeabilization
Yes - 1% TritonX-100
Specification
Cortex
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 27 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cortical)
Permeabilization
Yes - 1% TritonX-100
Specification
Cortical
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 27 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Cortical)
Permeabilization
Yes - 1% TritonX-100
Specification
Cortical
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 27 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (brain)
Permeabilization
Yes - 1% TritonX in PBS
Specification
brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 23 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain- hippocampal)
Permeabilization
Yes - 1% TritonX in PBS
Specification
Brain- hippocampal
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 23 2021

Immunocytochemistry abreview for Anti-MAP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (iPSC-NSC)
Permeabilization
Yes - 0.5% triton
Specification
iPSC-NSC
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 16 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (iPSCs derived human neurons)
Permeabilization
Yes - 0.5% Triton X100
Specification
iPSCs derived human neurons
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

HERR DR. Vladimir Milenkovic

Verified customer

Submitted Nov 30 2020

1-10 of 76 Abreviews or Q&A

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