Overview

  • Product name

  • Description

    Chicken polyclonal to MAP2
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Two synthetic peptidescorresponding to different regions of the MAP2 gene product, but shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences conjugated to KLH.

  • Positive control

    • Cultured neurons from an e13.5 mouse brain.
  • General notes


    Two different affinity purified anti peptide antibodies were combined to make this product. The concentrations of both of these antibodies was 100 µg/ml, making the total antibody concentration 200 µg/ml.

Properties

Applications

Our Abpromise guarantee covers the use of ab75713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 200 kDa.
IHC-P 1/1000 - 1/2000.
ICC/IF 1/1000 - 1/2000.

Target

  • Function

    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities

    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications

    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of cultured neonatal mouse cortex cells labelling MAP2 (green) with ab75713. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-GFAP.

  • Immunocytochemistry/Immunofluorescence analysis of cultured neonatal mouse cortex cells labelling MAP2 (green) with ab75713. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-GFAP, Blue - DAPI nuclear stain.

References

This product has been referenced in:

  • Huang X  et al. Non-invasive Low-Intensity Pulsed Ultrasound Modulates Primary Cilia of Rat Hippocampal Neurons. Ultrasound Med Biol 45:1274-1283 (2019). Read more (PubMed: 30795858) »
  • Zhu H  et al. GSK3ß-mediated tau hyperphosphorylation triggers diabetic retinal neurodegeneration by disrupting synaptic and mitochondrial functions. Mol Neurodegener 13:62 (2018). Read more (PubMed: 30466464) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us and thank you for submitting an abreview. The abreview program is only for testing primary antibodies in untested species/assays. Unfortunately it does not include secondary antibodies. However, it looks as the seconday antibody appears to work fine in your tests. This product, if stored correctly (aliquot and store at -20C in dark), will continue to perform for 5 years from the date of arrival. Although there are lesser numbers of chicken raised antibodies than those in other species, at this time we offer 805 primary antibodies that have been raised in chicken. Please feel free to contact me if you have any questions.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Acetone
Permeabilization
Yes - 0.3% Triton X-100/PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. Julie Nigro

Verified customer

Submitted Dec 28 2011

Answer

Thank you for submitting an Abreview of ab56164. As you may have noticed, your review has now been published on our website. We appreciate the time and effort that you have put into this and would like to express our gratitude. If there is anything that we may do to help you reach you research goals please do not hesitate to contact us.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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