Overview

  • Product name

  • Description

    Chicken polyclonal to MAP2
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Two synthetic peptidescorresponding to different regions of the MAP2 gene product, but shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences conjugated to KLH.

  • Positive control

    • Cultured neurons from an e13.5 mouse brain.
  • General notes


    Two different affinity purified anti peptide antibodies were combined to make this product. The concentrations of both of these antibodies was 100 µg/ml, making the total antibody concentration 200 µg/ml.

Properties

Applications

Our Abpromise guarantee covers the use of ab75713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 200 kDa.
IHC-P 1/1000 - 1/2000.
ICC/IF 1/1000 - 1/2000.

Target

  • Function

    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities

    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications

    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of cultured neonatal mouse cortex cells labelling MAP2 (green) with ab75713. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-GFAP.

  • Immunocytochemistry/Immunofluorescence analysis of cultured neonatal mouse cortex cells labelling MAP2 (green) with ab75713. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-GFAP, Blue - DAPI nuclear stain.

References

This product has been referenced in:

  • Huang X  et al. Non-invasive Low-Intensity Pulsed Ultrasound Modulates Primary Cilia of Rat Hippocampal Neurons. Ultrasound Med Biol 45:1274-1283 (2019). Read more (PubMed: 30795858) »
  • Zhu H  et al. GSK3ß-mediated tau hyperphosphorylation triggers diabetic retinal neurodegeneration by disrupting synaptic and mitochondrial functions. Mol Neurodegener 13:62 (2018). Read more (PubMed: 30466464) »
See all 3 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Acetone
Permeabilization
Yes - 0.3% Triton X-100/PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. Julie Nigro

Verified customer

Submitted Dec 28 2011

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