Other product details
Host species: Goat
Conjugation: Alexa Fluor® 568
The images show coronal sections of adult mouse brain fixed with either acetone (top panel) or methanol (bottom panel). The field of view is the intersection between the cortex hemispheres, showing major blood vessels. Because this antibody is staining cells around blood vessels rather than neuronal cells within the cortex, it is likely that it is staining glial cells like astrocytes which are located around blood vessels and function to maintain the integrity of the blood-brain-barrier.
In serial sections were an isotype control (chicken IgY), was used instead of the ab75713 (images on the right), the staining was negative indicating that the pattern of staining with ab75713 is specific for astrocytes.
Compared with methanol fixation, the staining intensity for ab75713 is better after acetone fixation. When mouse brain sections were fixed in 2%PFA/0.05% gluteraldehyde, there was no positive staining observed with ab75713 antibody, even around major blood vessels.
For future experiments we would recommend trying a different fixative, such as paraformaldehyde and possibly an antigen retrieval step.
Dr. Julie Nigro
Submitted Dec 28 2011