Anti-MAP2 antibody (ab92434)
Key features and details
- Chicken polyclonal to MAP2
- Suitable for: WB, ICC
- Reacts with: Rat, Human
- Isotype: IgY
Overview
-
Product name
Anti-MAP2 antibody
See all MAP2 primary antibodies -
Description
Chicken polyclonal to MAP2 -
Host species
Chicken -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein corresponding to Cow MAP2. Recombinant bovine MAP2 protein expressed in and purified from E. Coli
Database link: F1MEW3 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide -
Concentration information loading...
-
Purity
IgY fraction -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/20000. Predicted molecular weight: 280 kDa.
|
|
ICC | (1) |
Use at an assay dependent concentration.
|
Notes |
---|
WB
1/20000. Predicted molecular weight: 280 kDa. |
ICC
Use at an assay dependent concentration. |
Target
-
Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
Sequence similarities
Contains 3 Tau/MAP repeats. -
Post-translational
modificationsPhosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 4133 Human
- Entrez Gene: 25595 Rat
- Omim: 157130 Human
- SwissProt: P11137 Human
- SwissProt: P15146 Rat
- Unigene: 368281 Human
- Unigene: 10484 Rat
- Unigene: 136301 Rat
see all -
Alternative names
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
Images
-
ab92434 staining MAP2 in mouse brain neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2500 in PBS) for 24 hours at 4°C. ab150169, a goat anti-chicken Alexa Fluor® 488 (1/500) was used as the secondary antibody.
-
Anti-MAP2 antibody (ab92434) at 1/20000 dilution + Rat cerebral cortex lysate
Predicted band size: 280 kDa -
ab92434 at 1/2500 dilution staining MAP2 in mixed neuron/glial cultures by Immunofluorescence. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.
Datasheets and documents
-
SDS download
-
Datasheet download
References (45)
ab92434 has been referenced in 45 publications.
- Kim YA et al. RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation. Acta Neuropathol 145:29-48 (2023). PubMed: 36357715
- Ryan SK et al. Microglia ferroptosis is regulated by SEC24B and contributes to neurodegeneration. Nat Neurosci 26:12-26 (2023). PubMed: 36536241
- Mishra S et al. The Alzheimer's gene SORL1 is a regulator of endosomal traffic and recycling in human neurons. Cell Mol Life Sci 79:162 (2022). PubMed: 35226190
- Azevedo C et al. Parkinson's disease and multiple system atrophy patient iPSC-derived oligodendrocytes exhibit alpha-synuclein-induced changes in maturation and immune reactive properties. Proc Natl Acad Sci U S A 119:e2111405119 (2022). PubMed: 35294277
- Teng Z & Gottmann K Hemisynapse Formation Between Target Astrocytes and Cortical Neuron Axons in vitro. Front Mol Neurosci 15:829506 (2022). PubMed: 35386271