Recombinant Anti-MAP2 antibody [EPR22036-127] (ab221693)
![Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22036-127] (ab221693)](https://www.abcam.com/ps/products/221/ab221693/Images/ab221693-316992-anti-map2-antibody-epr22036-127-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22036-127] (ab221693)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22036-127] to MAP2
- Suitable for: IHC-Fr, ICC/IF
- Reacts with: Mouse
Overview
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Product name
Anti-MAP2 antibody [EPR22036-127]
See all MAP2 primary antibodies -
Description
Rabbit monoclonal [EPR22036-127] to MAP2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseIHC-Fr Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-Fr: Mouse cerebrum, kidney and liver tissue. ICC/IF: Neuro-2a cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22036-127 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Mouse
|
| IHC-Fr |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/1000.
|
|
| ICC/IF |
1/1000.
|
| Notes |
|---|
|
IHC-Fr
1/1000. |
|
ICC/IF
1/1000. |
Target
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Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
Sequence similarities
Contains 3 Tau/MAP repeats. -
Post-translational
modificationsPhosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 17756 Mouse
- SwissProt: P20357 Mouse
- Unigene: 256966 Mouse
- Unigene: 436793 Mouse
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Alternative names
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22036-127] (ab221693)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling MAP2 with ab221693 at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cells is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control: Hepa1-6 cells (PMID: 7001466)
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![Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)](https://www.abcam.com/ps/products/221/ab221693/Images/ab221693-316993-anti-map2-antibody-epr22036-127-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse cerebrum tissue labeling MAP2 (Green) using ab221693 at 1/1000 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Cytoplasmic staining on mouse cerebrum (PMID: 22479190) is observed. The nuclear counterstain is DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)](https://www.abcam.com/ps/products/221/ab221693/Images/ab221693-316994-anti-map2-antibody-epr22036-127-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse kidney tissue labeling MAP2 using ab221693 at 1/1000 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Negative control: No staining on mouse kidney (PMID: 7001466, PMID: 2423532) is observed. The nuclear counterstain is DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)](https://www.abcam.com/ps/products/221/ab221693/Images/ab221693-316995-anti-map2-antibody-epr22036-127-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse liver tissue labeling MAP2 using ab221693 at 1/1000 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Negative control: No staining on mouse liver (PMID: 7001466, PMID: 2423532). is observed. The nuclear counterstain is DAPI (Blue).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Anti-MAP2 antibody [EPR22036-127] (ab221693)](https://www.abcam.com/ps/products/221/ab221693/Images/ab221693-5-benefits-of-recombinant-antibodies.png)
Anti-MAP2 antibody [EPR22036-127] (ab221693)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab221693 has not yet been referenced specifically in any publications.