Recombinant
RabMAb

Recombinant Anti-MAP2 antibody [EPR22036-127] (ab221693)

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Overview

  • Product name

    Anti-MAP2 antibody [EPR22036-127]
    See all MAP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22036-127] to MAP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment within Mouse MAP2 aa 1-500. The exact sequence is proprietary.
    Database link: P20357

  • Positive control

    • IHC-Fr: Mouse cerebrum, kidney and liver tissue. ICC/IF: Neuro-2a cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab221693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1000.
ICC/IF 1/1000.

Target

  • Function

    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

  • Sequence similarities

    Contains 3 Tau/MAP repeats.

  • Post-translational
    modifications

    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.

  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • DKFZp686I2148 antibody
      • MAP 2 antibody
      • MAP dendrite specific antibody
      • MAP-2 antibody
      • MAP2 antibody
      • MAP2A antibody
      • MAP2B antibody
      • MAP2C antibody
      • Microtubule associated protein 2 antibody
      • Microtubule-associated protein 2 antibody
      • MTAP2_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22036-127] (ab221693)
      Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22036-127] (ab221693)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling MAP2 with ab221693 at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cells is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Negative control: Hepa1-6 cells (PMID: 7001466)

    • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)
      Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)

      Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse cerebrum tissue labeling MAP2 (Green) using ab221693 at 1/1000 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Cytoplasmic staining on mouse cerebrum (PMID: 22479190) is observed. The nuclear counterstain is DAPI (Blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

       

    • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)
      Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)

      Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse kidney tissue labeling MAP2 using ab221693 at 1/1000 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Negative control: No staining on mouse kidney (PMID: 7001466, PMID: 2423532) is observed. The nuclear counterstain is DAPI (Blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

       

    • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)
      Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] (ab221693)

      Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse liver tissue labeling MAP2 using ab221693 at 1/1000 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Negative control: No staining on mouse liver (PMID: 7001466, PMID: 2423532). is observed. The nuclear counterstain is DAPI (Blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

       

    References

    ab221693 has not yet been referenced specifically in any publications.

    Publishing research using ab221693? Please let us know so that we can cite the reference in this datasheet.

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