Recombinant
RabMAb

Recombinant Anti-MAP2 antibody [EPR22036-127] - BSA and Azide free (ab238889)

Submit a review Submit a question

Overview

  • Product name

    Anti-MAP2 antibody [EPR22036-127] - BSA and Azide free
    See all MAP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22036-127] to MAP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment within Mouse MAP2 aa 1-500. The exact sequence is proprietary.
    Database link: P20357

  • Positive control

    • IHC-Fr: Mouse cerebrum tissue.

  • General notes

    Ab238889 is the carrier-free version of ab221693. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238889 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

  • Sequence similarities

    Contains 3 Tau/MAP repeats.

  • Post-translational
    modifications

    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.

  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • DKFZp686I2148 antibody
      • MAP 2 antibody
      • MAP dendrite specific antibody
      • MAP-2 antibody
      • MAP2 antibody
      • MAP2A antibody
      • MAP2B antibody
      • MAP2C antibody
      • Microtubule associated protein 2 antibody
      • Microtubule-associated protein 2 antibody
      • MTAP2_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] - BSA and Azide free (ab238889)
      Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] - BSA and Azide free (ab238889)

      Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse liver tissue labeling MAP2 using ab221693 at 1/1000 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Negative control: No staining on mouse liver (PMID: 7001466, PMID: 2423532). is observed. The nuclear counterstain is DAPI (Blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221693).

    • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] - BSA and Azide free (ab238889)
      Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] - BSA and Azide free (ab238889)

      Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse kidney tissue labeling MAP2 using ab221693 at 1/1000 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Negative control: No staining on mouse kidney (PMID: 7001466, PMID: 2423532) is observed. The nuclear counterstain is DAPI (Blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221693).

    • Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22036-127] - BSA and Azide free (ab238889)
      Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22036-127] - BSA and Azide free (ab238889)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling MAP2 with ab221693 at 1/1000 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cells is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as a counterstain at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Negative control: Hepa1-6 cells (PMID: 7001466)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221693).

    • Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] - BSA and Azide free (ab238889)
      Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] - BSA and Azide free (ab238889)

      Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse cerebrum tissue labeling MAP2 (Green) using ab221693 at 1/1000 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Cytoplasmic staining on mouse cerebrum (PMID: 22479190) is observed. The nuclear counterstain is DAPI (Blue).

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab221693).

    References

    ab238889 has not yet been referenced specifically in any publications.

    Publishing research using ab238889? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab238889.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com