Recombinant Anti-MAP2 antibody [EPR22641-16] (ab254264)
![Immunocytochemistry - Anti-MAP2 antibody [EPR22641-16] (ab254264)](https://www.abcam.com/ps/products/254/ab254264/Images/ab254264-10-anti-map2-antibody-epr22641-16-immunocytochemistry-mouse-primary-neuralglia-cell.jpg "Immunocytochemistry - Anti-MAP2 antibody [EPR22641-16] (ab254264)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22641-16] to MAP2
- Suitable for: IHC-Fr, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MAP2 antibody [EPR22641-16]
See all MAP2 primary antibodies -
Description
Rabbit monoclonal [EPR22641-16] to MAP2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt,IP or WB -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Common marmoset
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: mouse primary neural/glia cell.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22641-16 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/500.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
| ICC/IF |
1/2000.
|
|
| IHC-P | (4) |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IHC-Fr
1/500. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
ICC/IF
1/2000. |
|
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
Sequence similarities
Contains 3 Tau/MAP repeats. -
Post-translational
modificationsPhosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 4133 Human
- Entrez Gene: 17756 Mouse
- Entrez Gene: 25595 Rat
- Omim: 157130 Human
- SwissProt: P11137 Human
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 368281 Human
see all -
Alternative names
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
Images
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Immunocytochemistry - Anti-MAP2 antibody [EPR22641-16] (ab254264)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling MAP2 with ab254264 at 1/2000 (0.299 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2ug/ml dilution.
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![Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)](https://www.abcam.com/ps/products/254/ab254264/Images/ab254264-8-anti-map2-epr22641-16-immunohistochemistry-frozen-cerebral-cortex-rat.jpg)
Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebral cortex tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on rat cerebral cortex (PMID: 22275884) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)](https://www.abcam.com/ps/products/254/ab254264/Images/ab254264-7-anti-map2-epr22641-16-immunohistochemistry-frozen-cerebral-cortex-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebral cortex tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on mouse cerebral cortex (PMID: 22275884) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)](https://www.abcam.com/ps/products/254/ab254264/Images/ab254264-6-anti-map2-epr22641-16-immunohistochemistry-frozen-cerebellum-rat.jpg)
Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on rat cerebellum (PMID: 22275884) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)](https://www.abcam.com/ps/products/254/ab254264/Images/ab254264-5-anti-map2-epr22641-16-immunohistochemistry-frozen-cerebellum-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on mouse cerebellum (PMID: 22275884) is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)](https://www.abcam.com/ps/products/254/ab254264/Images/ab254264-4-anti-map2-antibody-epr22641-16-immunohistochemistry-colon-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in neuron of human colon (PMID: 28766965,15642108) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)](https://www.abcam.com/ps/products/254/ab254264/Images/ab254264-3-anti-map2-antibody-epr22641-16-immunohistochemistry-cerebral-cortex-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in rat cerebrum (PMID: 28766965,15642108) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)](https://www.abcam.com/ps/products/254/ab254264/Images/ab254264-2-anti-map2-antibody-epr22641-16-immunohistochemistry-cerebral-cortex-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in mouse cerebrum (PMID: 28766965,15642108) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)](https://www.abcam.com/ps/products/254/ab254264/Images/ab254264-1-anti-map2-antibody-epr22641-16-immunohistochemistry-cerebral-cortex-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] (ab254264)Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human cerebrum (PMID: 28766965,15642108) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
-
![Anti-MAP2 antibody [EPR22641-16] (ab254264)](https://www.abcam.com/ps/products/254/ab254264/Images/ab254264-9-benefits-of-recombinant-antibodies.png)
Anti-MAP2 antibody [EPR22641-16] (ab254264)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab254264 has been referenced in 2 publications.
- Zheng Y et al. Spatial learning and memory deficits induced by prenatal glucocorticoid exposure depend on hippocampal CRHR1 and CXCL5 signaling in rats. J Neuroinflammation 18:85 (2021). PubMed: 33810797
- Chen C et al. RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation. Aging (Albany NY) 12:14490-14505 (2020). PubMed: 32693388
