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Other Immunogen Type corresponding to MAP2. HM-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rat brain microtubule associated proteins (MAPs).
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Our Abpromise guarantee covers the use of ab11267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||1/500. Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .|
|IHC-FrFl||Use at an assay dependent concentration. PubMed: 24223856|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 280 kDa. (see Farah et al).|
|IHC-P||Use at an assay dependent concentration.|
|IHC-FoFr||1/500. PubMed: 20424326|
ab11267 staining MAP2 in human hippocampal progenitor cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 5% serum for 1 hour at 18°C. Samples were incubated with the undiluted primary antibody for 12 hours at 4°C. An undiluted Alexa Fluor® 555-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody.
Immunocytochemical analysis of B35 cells labelling MAP2 with ab11267 at a concentration of 2 μg/mL. The secondary was developed using Goat anti-mouse IgG. Cells were counterstained with DAPI to stain nuclei.
ab11267 staining rat brain tissue sections by IHC-Fr. Sections were PFA fixed and permeabilized in TritonX100 prior to blocking with 3% BSA for 1 hour. The rpimary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® conjugated goat anti-mouse was used as the secondary antibody.
The image shows a cross section through the rat hipocampal CA1 area at magnification 200x . The anti-MAP2 staining is clearly visible in dendrites of pyramidal cells.
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