Overview

  • Product name
    Anti-MAP2 antibody [HM-2]
    See all MAP2 primary antibodies
  • Description
    Mouse monoclonal [HM-2] to MAP2
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC, IHC-Fr, ICC/IF, IHC-FrFl, WB, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human, Quail
  • Immunogen

    Other Immunogen Type corresponding to MAP2. HM-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rat brain microtubule associated proteins (MAPs).

  • Positive control
    • Rat brain extract.
  • General notes

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab11267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/500. Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .
IHC-FrFl Use at an assay dependent concentration. PubMed: 24223856
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 280 kDa. (see Farah et al).
IHC-P Use at an assay dependent concentration.
IHC-FoFr 1/500. PubMed: 20424326

Target

  • Function
    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities
    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications
    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Images

  • Rivastigmine preserves neuronal morphology and alters sAPP secretion

    At day 12 or day 20, cells were fixed for immunocytochemistry analysis and probed with anti-MAP2 (green) and anti-GFAP (red) antibodies. Neuronal MAP2 immunoreactivity declines to almost undetectable levels between day 12 and day 20 in untreated cells, whereas neuronal morphology is preserved in rivastigmine treated cultures. Glial GFAP was observed to increase in both treated and untreated cells between day 12 and day 20. These results confirm the degeneration of neurons by day 20.

    MAP2 is detected using ab11267 in 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized embryonic rat cerebrocortical cells.

    (From Figure 2A of Bailey et al)

  • Immunocytochemical analysis of B35 cells labelling MAP2 with ab11267 at a concentration of 2 μg/mL. The secondary was developed using Goat anti-mouse IgG. Cells were counterstained with DAPI to stain nuclei.

  • Anti-MAP2 antibody [HM-2] (ab11267) at 1 µg/ml + Rat brain lysate
  • ab11267 staining rat brain tissue sections by IHC-Fr.  Sections were PFA fixed and permeabilized in TritonX100 prior to blocking with 3% BSA for 1 hour.  The rpimary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C.  An Alexa Fluor® conjugated goat anti-mouse was used as the secondary antibody. 

    The image shows a cross section through the rat hipocampal CA1 area at magnification 200x . The anti-MAP2 staining is clearly visible in dendrites of pyramidal cells.

    See Abreview

  • ab11267 staining MAP2 in human hippocampal progenitor cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 5% serum for 1 hour at 18°C. Samples were incubated with the undiluted primary antibody for 12 hours at 4°C. An undiluted Alexa Fluor® 555-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Paik EJ  et al. Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hIPSC culture. Sci Rep 8:804 (2018). Read more (PubMed: 29339826) »
  • Riquelme D  et al. Subcellular Localization and Activity of TRPM4 in Medial Prefrontal Cortex Layer 2/3. Front Cell Neurosci 12:12 (2018). Read more (PubMed: 29440991) »
See all 73 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Rat Cell (Cortical neurons from E17 embryos)
Permeabilization
Yes - 0.2% Triton X 5 mins
Specification
Cortical neurons from E17 embryos
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 12 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Neuroblastoma (Neuro2A))
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
400000 cells
Treatment
10% FBS and fibroblast growth factor
Specification
Mouse Neuroblastoma (Neuro2A)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Abcam user community

Verified customer

Submitted Apr 27 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Sample
Human Cell (hippocampal progenitor cells)
Specification
hippocampal progenitor cells
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Dr. Aleksandra Maruszak

Verified customer

Submitted Mar 31 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Neural stem cells and progenitors)
Specification
Neural stem cells and progenitors
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton-X-100 for 30 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 02 2012

Answer

I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.  

Read More

Answer

Thanks for your reply. I am very sorry these anti-GFAP and anti-MAP2 antibodies continue to give you trouble. I'd be happy to send you any two replacement antibodies from our catalog. Or if you prefer I can set up a credit or refund instead. Please let me know how you would like to proceed.

Read More

Question

to whom may be concerned: our lab ordered four antibodies from your company in July. we cannot find the order list now. ab90601 sheep polyconal to GFAP. ab11267 mouse monoclonal HM-2 to MAP2 - neuronal marker and other two secondary antibodies: ab6896 Donkey polyclonal secondary antibody to sheep IgG - H&L(FITC) and ab47052 Goat polyclonal secondary antibody to Mouse IgG - H&L(AMCA). we used the GFAP to stain astrocyte of rat brain frozen sections and MAP2 to stain neuron of rat brain frozen sections. However, we found the images of GFAP staining were very similar to neurons, clearly not astrocytes. we repeat experiment at least three times and obtain the same results. last time, we borrowed GFAP antibody from other lab ( DAKO company product ) and followed the same procedures and make comparison with your company's GFAP. We obtained typical image of astrocyte by GFAP of borrowed antibody (DAKO product). However, for your company's GFAP, we obtained images similar to neurons just like before. Besides, for the MAP2 of your company, we cannot get any typical images of neurons although we followed the same procedures as doing GFAP. For every experiment, we made negative controls. Finally, we believe there must be somthing wrong with your two primary antibodies(GFAP and MAP2). It cost us more than two weeks to do such experiments which made us very frustrated. We just want to proceed our experiment quickly. so IF possible, someone can check it for us and send us the right antibody quickly so we can continue our experiments.

Read More
Answer

We spoke just a short while ago regarding these issues you were having with ab90601 and ab11267. I have processed the replacement orders as we discussed and sent you an update via email just a few minutes ago. You should receive these 2 replacement antibodies for ab90601 and ab11267 tomorrow. I am sorry for the trouble these 2 antibodies have caused and I do hope the replacements work as expected. Please feel free to follow up with me if you need anything further.

Read More

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab90601 and one vial of ab11267. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Read More

Answer

Thank you for contacting us. I am sorry that ab11267 did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. Regarding ab109506, I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.   I wish you the best of luck with your research.  

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Adult C57BL/6 mouse coronal brain sections)
Specification
Adult C57BL/6 mouse coronal brain sections

Mr. Xiao-Dong Wang

Verified customer

Submitted Aug 03 2010

1-10 of 13 Abreviews or Q&A

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