Overview

  • Product name
    Anti-MAP2 antibody [HM-2]
    See all MAP2 primary antibodies
  • Description
    Mouse monoclonal [HM-2] to MAP2
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC, IHC-Fr, ICC/IF, IHC-FrFl, WB, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human, Quail
  • Immunogen

    Other Immunogen Type corresponding to MAP2. HM-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rat brain microtubule associated proteins (MAPs).

  • Positive control
    • Rat brain extract.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 05/06/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab11267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .

IHC-FrFl Use at an assay dependent concentration. PubMed: 24223856
WB Use at an assay dependent concentration. Detects a band of approximately 280 kDa.
IHC-P Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20424326

Target

  • Function
    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities
    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications
    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Images

  • Rivastigmine preserves neuronal morphology and alters sAPP secretion

    At day 12 or day 20, cells were fixed for immunocytochemistry analysis and probed with anti-MAP2 (green) and anti-GFAP (red) antibodies. Neuronal MAP2 immunoreactivity declines to almost undetectable levels between day 12 and day 20 in untreated cells, whereas neuronal morphology is preserved in rivastigmine treated cultures. Glial GFAP was observed to increase in both treated and untreated cells between day 12 and day 20. These results confirm the degeneration of neurons by day 20.

    MAP2 is detected using ab11267 in 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized embryonic rat cerebrocortical cells.

    (From Figure 2A of Bailey et al)

    This image was generated using the ascites version of the product.

  • Immunocytochemical analysis of B35 cells labelling MAP2 with ab11267 at a concentration of 2 μg/mL. The secondary was developed using Goat anti-mouse IgG. Cells were counterstained with DAPI to stain nuclei.

    This image was generated using the ascites version of the product.

  • Anti-MAP2 antibody [HM-2] (ab11267) at 1 µg/ml + Rat brain lysate


    This image was generated using the ascites version of the product.

  • ab11267 staining rat brain tissue sections by IHC-Fr.  Sections were PFA fixed and permeabilized in TritonX100 prior to blocking with 3% BSA for 1 hour.  The rpimary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C.  An Alexa Fluor® conjugated goat anti-mouse was used as the secondary antibody. 

    The image shows a cross section through the rat hipocampal CA1 area at magnification 200x . The anti-MAP2 staining is clearly visible in dendrites of pyramidal cells.

    This image was generated using the ascites version of the product.

    See Abreview

  • ab11267 staining MAP2 in human hippocampal progenitor cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 5% serum for 1 hour at 18°C. Samples were incubated with the undiluted primary antibody for 12 hours at 4°C. An undiluted Alexa Fluor® 555-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    This image was generated using the ascites version of the product.

    See Abreview

References

This product has been referenced in:
  • Li Z  et al. APC-Cdh1 Regulates Neuronal Apoptosis Through Modulating Glycolysis and Pentose-Phosphate Pathway After Oxygen-Glucose Deprivation and Reperfusion. Cell Mol Neurobiol 39:123-135 (2019). Read more (PubMed: 30460429) »
  • Lim GS  et al. Microhexagon gradient array directs spatial diversification of spinal motor neurons. Theranostics 9:311-323 (2019). Read more (PubMed: 30809276) »
See all 81 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-6 of 6 Abreviews

Application
Immunocytochemistry
Sample
Rat Cell (Cortical neurons from E17 embryos)
Permeabilization
Yes - 0.2% Triton X 5 mins
Specification
Cortical neurons from E17 embryos
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 12 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Neuroblastoma (Neuro2A))
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
400000 cells
Treatment
10% FBS and fibroblast growth factor
Specification
Mouse Neuroblastoma (Neuro2A)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C

Abcam user community

Verified customer

Submitted Apr 27 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Sample
Human Cell (hippocampal progenitor cells)
Specification
hippocampal progenitor cells
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Dr. Aleksandra Maruszak

Verified customer

Submitted Mar 31 2014

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Neural stem cells and progenitors)
Specification
Neural stem cells and progenitors
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton-X-100 for 30 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 02 2012

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Adult C57BL/6 mouse coronal brain sections)
Specification
Adult C57BL/6 mouse coronal brain sections

Mr. Xiao-Dong Wang

Verified customer

Submitted Aug 03 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - TritonX100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%

Dr. Grazyna Niewiadomska

Verified customer

Submitted Nov 23 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up