For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    map2-antibody-hm-2-ab11267.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Cell Type Marker Neuron marker Soma marker
Share by email

Anti-MAP2 antibody [HM-2] (ab11267)

  • Datasheet
  • SDS
Reviews (8)Q&A (7)References (133)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)
  • Western blot - Anti-MAP2 antibody [HM-2] (ab11267)
  • Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)

Key features and details

  • Mouse monoclonal [HM-2] to MAP2
  • Suitable for: ICC, WB
  • Reacts with: Rat
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Human MAP2 protein (ab114686)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Primary
Product image
Anti-MAP2 (phospho S136) antibody [EPR2361] - BSA and Azide free (ab247601)

View more associated products

Overview

  • Product name

    Anti-MAP2 antibody [HM-2]
    See all MAP2 primary antibodies
  • Description

    Mouse monoclonal [HM-2] to MAP2
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC, WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Chicken, Cow, Human, Quail
  • Immunogen

    Full length protein corresponding to Rat MAP2. Rat brain microtubule associated proteins (MAPs).

  • Positive control

    • WB: Rat brain tissue lysate. ICC: B35 cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 05/06/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    HM-2
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Dendrite marker
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human MAP2 protein (ab114686)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Rat MAP2 peptide (ab32453)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC (2)
Use a concentration of 2 µg/ml.

Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .

WB (2)
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 200 kDa.
Notes
ICC
Use a concentration of 2 µg/ml.

Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .

WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 200 kDa.

Target

  • Function

    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities

    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications

    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Target information above from: UniProt accession P11137 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4133 Human
    • Entrez Gene: 17756 Mouse
    • Entrez Gene: 25595 Rat
    • Omim: 157130 Human
    • SwissProt: P11137 Human
    • SwissProt: P20357 Mouse
    • SwissProt: P15146 Rat
    • Unigene: 368281 Human
    • Unigene: 256966 Mouse
    • Unigene: 436793 Mouse
    • Unigene: 10484 Rat
    • Unigene: 136301 Rat
    • Unigene: 230443 Rat
    see all
  • Alternative names

    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Images

  • Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)
    Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)Bailey, J.A. et al PLoS One. 2011;6(7):e21954. doi: 10.1371/journal.pone.0021954. Epub 2011 Jul 22 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/

    Rivastigmine preserves neuronal morphology and alters sAPP secretion

    At day 12 or day 20, cells were fixed for immunocytochemistry analysis and probed with anti-MAP2 (green) and anti-GFAP (red) antibodies. Neuronal MAP2 immunoreactivity declines to almost undetectable levels between day 12 and day 20 in untreated cells, whereas neuronal morphology is preserved in rivastigmine treated cultures. Glial GFAP was observed to increase in both treated and untreated cells between day 12 and day 20. These results confirm the degeneration of neurons by day 20.

    MAP2 is detected using ab11267 in 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized embryonic rat cerebrocortical cells.

    (From Figure 2A of Bailey et al)

    This image was generated using the ascites version of the product.

  • Western blot - Anti-MAP2 antibody [HM-2] (ab11267)
    Western blot - Anti-MAP2 antibody [HM-2] (ab11267)
    Anti-MAP2 antibody [HM-2] (ab11267) at 1 µg/ml + Rat brain lysate

    Predicted band size: 200 kDa



    This image was generated using the ascites version of the product.

  • Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)
    Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)

    Immunocytochemical analysis of B35 (rat neuroblastoma cell line) cells labelling MAP2 with ab11267 at a concentration of 2 μg/mL. The secondary was developed using Goat anti-mouse IgG. Cells were counterstained with DAPI to stain nuclei.

    This image was generated using the ascites version of the product.

Protocols

  • Recommended IP protocol with ab11267
  • Recommended IHC protocols with ab11267

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (133)

Publishing research using ab11267? Please let us know so that we can cite the reference in this datasheet.

ab11267 has been referenced in 133 publications.

  • Grubisha MJ  et al. MAP2 is differentially phosphorylated in schizophrenia, altering its function. Mol Psychiatry N/A:N/A (2021). PubMed: 33526823
  • Varinthra P  et al. The 4-(Phenylsulfanyl) butan-2-one Improves Impaired Fear Memory Retrieval and Reduces Excessive Inflammatory Response in Triple Transgenic Alzheimer's Disease Mice. Front Aging Neurosci 13:615079 (2021). PubMed: 33613267
  • Choi GE  et al. BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects. Nat Commun 12:487 (2021). PubMed: 33473105
  • Cascella R  et al. The release of toxic oligomers from a-synuclein fibrils induces dysfunction in neuronal cells. Nat Commun 12:1814 (2021). PubMed: 33753734
  • Wang H  et al. Mettl14-mediated m6A modification modulates neuron apoptosis during the repair of spinal cord injury by regulating the transformation from pri-mir-375 to miR-375. Cell Biosci 11:52 (2021). PubMed: 33706799
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review

Filter by Application

Filter by Species

Filter by Ratings

1-8 of 8 Abreviews

Western blot abreview for Anti-MAP2 antibody [HM-2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Read More

DR. Alexandre Magno

Verified customer

Submitted Dec 06 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody [HM-2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (brain)
Permeabilization
Yes - 0.3% Triton-X-100 for 15 min
Specification
brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

DR. Uthra Rajamani

Verified customer

Submitted Jan 22 2021

Immunocytochemistry abreview for Anti-MAP2 antibody [HM-2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Rat Cell (Cortical neurons from E17 embryos)
Permeabilization
Yes - 0.2% Triton X 5 mins
Specification
Cortical neurons from E17 embryos
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 12 2015

Western blot abreview for Anti-MAP2 antibody [HM-2]

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Neuroblastoma (Neuro2A))
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
400000 cells
Treatment
10% FBS and fibroblast growth factor
Specification
Mouse Neuroblastoma (Neuro2A)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Read More

Abcam user community

Verified customer

Submitted Apr 27 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-MAP2 antibody [HM-2] - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Sample
Human Cell (hippocampal progenitor cells)
Specification
hippocampal progenitor cells
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Read More

Dr. Aleksandra Maruszak

Verified customer

Submitted Mar 31 2014

Immunocytochemistry abreview for Anti-MAP2 antibody [HM-2] - Neuronal Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Neural stem cells and progenitors)
Specification
Neural stem cells and progenitors
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton-X-100 for 30 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Nov 02 2012

Immunohistochemistry - Free Floating abreview for Anti-MAP2 antibody [HM-2] - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Adult C57BL/6 mouse coronal brain sections)
Specification
Adult C57BL/6 mouse coronal brain sections
Read More

Mr. Xiao-Dong Wang

Verified customer

Submitted Aug 03 2010

Immunohistochemistry (Frozen sections) abreview for Anti-MAP2 antibody [HM-2] - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - TritonX100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Read More

DR. Grazyna Niewiadomska

Verified customer

Submitted Nov 23 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.