Anti-MAP2 antibody [HM-2] (ab11267)
- Datasheet
- References (81)
- Protocols
Overview
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Product nameAnti-MAP2 antibody [HM-2]
See all MAP2 primary antibodies -
DescriptionMouse monoclonal [HM-2] to MAP2
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Host speciesMouse
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Tested applicationsSuitable for: ICC, IHC-Fr, ICC/IF, IHC-FrFl, WB, IHC-P, IHC-FoFrmore details
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Species reactivityReacts with: Mouse, Rat, Chicken, Cow, Human, Quail
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Immunogen
Other Immunogen Type corresponding to MAP2. HM-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rat brain microtubule associated proteins (MAPs).
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Positive control
- Rat brain extract.
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General notes
This product was changed from ascites to tissue culture supernatant on 05/06/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberHM-2
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab11267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) . |
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| IHC-FrFl | Use at an assay dependent concentration. PubMed: 24223856 | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 280 kDa. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 20424326 |
Target
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FunctionThe exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
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Sequence similaritiesContains 3 Tau/MAP repeats.
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Post-translational
modificationsPhosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
Cellular localizationCytoplasm, cytoskeleton.
- Information by UniProt
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Database links
- Entrez Gene: 4133 Human
- Entrez Gene: 17756 Mouse
- Entrez Gene: 25595 Rat
- Omim: 157130 Human
- SwissProt: P11137 Human
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 368281 Human
see all -
Alternative names
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)](https://www.abcam.com/ps/products/11/ab11267/Images/ab11267-320126-anti-map2-antibody-hm-2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)Bailey, J.A. et al PLoS One. 2011;6(7):e21954. doi: 10.1371/journal.pone.0021954. Epub 2011 Jul 22Rivastigmine preserves neuronal morphology and alters sAPP secretion
At day 12 or day 20, cells were fixed for immunocytochemistry analysis and probed with anti-MAP2 (green) and anti-GFAP (red) antibodies. Neuronal MAP2 immunoreactivity declines to almost undetectable levels between day 12 and day 20 in untreated cells, whereas neuronal morphology is preserved in rivastigmine treated cultures. Glial GFAP was observed to increase in both treated and untreated cells between day 12 and day 20. These results confirm the degeneration of neurons by day 20.
MAP2 is detected using ab11267 in 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized embryonic rat cerebrocortical cells.
(From Figure 2A of Bailey et al)
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)](https://www.abcam.com/ps/products/11/ab11267/Images/ab11267-251913-anti-map2-antibody-hm-2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)Immunocytochemical analysis of B35 cells labelling MAP2 with ab11267 at a concentration of 2 μg/mL. The secondary was developed using Goat anti-mouse IgG. Cells were counterstained with DAPI to stain nuclei.
This image was generated using the ascites version of the product.
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![Western blot - Anti-MAP2 antibody [HM-2] (ab11267)](https://www.abcam.com/ps/products/11/ab11267/Images/ab11267-320127-267b.jpg)
Western blot - Anti-MAP2 antibody [HM-2] (ab11267)Anti-MAP2 antibody [HM-2] (ab11267) at 1 µg/ml + Rat brain lysateThis image was generated using the ascites version of the product.
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![Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [HM-2] (ab11267)](https://www.abcam.com/ps/products/11/ab11267/Images/ab11267-18864-anti-map2-antibody-hm-2-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [HM-2] (ab11267)This image is courtesy of an Abreview submitted by Dr Grazyna Niewiadomskaab11267 staining rat brain tissue sections by IHC-Fr. Sections were PFA fixed and permeabilized in TritonX100 prior to blocking with 3% BSA for 1 hour. The rpimary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® conjugated goat anti-mouse was used as the secondary antibody.
The image shows a cross section through the rat hipocampal CA1 area at magnification 200x . The anti-MAP2 staining is clearly visible in dendrites of pyramidal cells.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)](https://www.abcam.com/ps/products/11/ab11267/Images/ab11267-220873-anti-map2-antibody-hm-2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [HM-2] (ab11267)This image is courtesy of an Abreview submitted by Aleksandra Maruszakab11267 staining MAP2 in human hippocampal progenitor cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 5% serum for 1 hour at 18°C. Samples were incubated with the undiluted primary antibody for 12 hours at 4°C. An undiluted Alexa Fluor® 555-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody.
This image was generated using the ascites version of the product.
References
This product has been referenced in:
- Li Z et al. APC-Cdh1 Regulates Neuronal Apoptosis Through Modulating Glycolysis and Pentose-Phosphate Pathway After Oxygen-Glucose Deprivation and Reperfusion. Cell Mol Neurobiol 39:123-135 (2019). Read more (PubMed: 30460429) »
- Lim GS et al. Microhexagon gradient array directs spatial diversification of spinal motor neurons. Theranostics 9:311-323 (2019). Read more (PubMed: 30809276) »
