Anti-MAP2 antibody [HM-2] (ab11267)
![Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)](https://www.abcam.com/ps/products/11/ab11267/Images/ab11267-320126-anti-map2-antibody-hm-2-immunofluorescence.jpg "Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)")
Key features and details
- Mouse monoclonal [HM-2] to MAP2
- Suitable for: ICC, WB
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-MAP2 antibody [HM-2]
See all MAP2 primary antibodies -
Description
Mouse monoclonal [HM-2] to MAP2 -
Host species
Mouse -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Chicken, Cow, Human, Quail
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Immunogen
Full length protein corresponding to Rat MAP2. Rat brain microtubule associated proteins (MAPs).
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Positive control
- WB: Rat brain tissue lysate. ICC: B35 cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 05/06/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
HM-2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | (2) |
Use a concentration of 2 µg/ml.
Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) . |
| WB | (2) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 200 kDa.
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| Notes |
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ICC
Use a concentration of 2 µg/ml. Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) . |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 200 kDa. |
Target
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Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
Sequence similarities
Contains 3 Tau/MAP repeats. -
Post-translational
modificationsPhosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 4133 Human
- Entrez Gene: 17756 Mouse
- Entrez Gene: 25595 Rat
- Omim: 157130 Human
- SwissProt: P11137 Human
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 368281 Human
see all -
Alternative names
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
Images
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Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)Bailey, J.A. et al PLoS One. 2011;6(7):e21954. doi: 10.1371/journal.pone.0021954. Epub 2011 Jul 22 Reproduced under the Creative Commons license https://creativecommons.org/publicdomain/zero/1.0/
Rivastigmine preserves neuronal morphology and alters sAPP secretion
At day 12 or day 20, cells were fixed for immunocytochemistry analysis and probed with anti-MAP2 (green) and anti-GFAP (red) antibodies. Neuronal MAP2 immunoreactivity declines to almost undetectable levels between day 12 and day 20 in untreated cells, whereas neuronal morphology is preserved in rivastigmine treated cultures. Glial GFAP was observed to increase in both treated and untreated cells between day 12 and day 20. These results confirm the degeneration of neurons by day 20.
MAP2 is detected using ab11267 in 4% paraformaldehyde-fixed, 0.5% Triton X-100 permeabilized embryonic rat cerebrocortical cells.
(From Figure 2A of Bailey et al)
This image was generated using the ascites version of the product.
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![Western blot - Anti-MAP2 antibody [HM-2] (ab11267)](https://www.abcam.com/ps/products/11/ab11267/Images/ab11267-320127-267b.jpg)
Western blot - Anti-MAP2 antibody [HM-2] (ab11267)Anti-MAP2 antibody [HM-2] (ab11267) at 1 µg/ml + Rat brain lysate
Predicted band size: 200 kDaThis image was generated using the ascites version of the product.
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![Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)](https://www.abcam.com/ps/products/11/ab11267/Images/ab11267-251913-anti-map2-antibody-hm-2-immunofluorescence.jpg)
Immunocytochemistry - Anti-MAP2 antibody [HM-2] (ab11267)Immunocytochemical analysis of B35 (rat neuroblastoma cell line) cells labelling MAP2 with ab11267 at a concentration of 2 μg/mL. The secondary was developed using Goat anti-mouse IgG. Cells were counterstained with DAPI to stain nuclei.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (133)
ab11267 has been referenced in 133 publications.
- Grubisha MJ et al. MAP2 is differentially phosphorylated in schizophrenia, altering its function. Mol Psychiatry N/A:N/A (2021). PubMed: 33526823
- Varinthra P et al. The 4-(Phenylsulfanyl) butan-2-one Improves Impaired Fear Memory Retrieval and Reduces Excessive Inflammatory Response in Triple Transgenic Alzheimer's Disease Mice. Front Aging Neurosci 13:615079 (2021). PubMed: 33613267
- Choi GE et al. BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects. Nat Commun 12:487 (2021). PubMed: 33473105
- Cascella R et al. The release of toxic oligomers from a-synuclein fibrils induces dysfunction in neuronal cells. Nat Commun 12:1814 (2021). PubMed: 33753734
- Wang H et al. Mettl14-mediated m6A modification modulates neuron apoptosis during the repair of spinal cord injury by regulating the transformation from pri-mir-375 to miR-375. Cell Biosci 11:52 (2021). PubMed: 33706799