Recombinant Anti-MAP2 antibody [RM1010] - BSA and Azide free (ab283865)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [RM1010] to MAP2 - BSA and Azide free
- Suitable for: WB, IHC-Fr, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MAP2 antibody [RM1010] - BSA and Azide free
See all MAP2 primary antibodies -
Description
Rabbit recombinant multiclonal [RM1010] to MAP2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, Flow Cyt (Intra), ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers. -
Positive control
- WB: SK-N-BE, IMR-32, Neuro-2a and PC-12 whole cell lysate; Mouse E12.5 brain, brain and cerebellum tissue lysates; Rat brain and cerebellum tissue lysates. IHC-Fr: Mouse cerebellum tissue; Rat cerebellum tissue. ICC/IF: Mouse primary neural/glia cells. Flow Cyt (intra): Mouse primary neuron cells; Neuro-2a cells.
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General notes
ab283865 is the carrier-free version of ab281588.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
RM1010 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab283865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 199 kDa.
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
|
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 199 kDa. |
IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
Sequence similarities
Contains 3 Tau/MAP repeats. -
Post-translational
modificationsPhosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
Cellular localization
Cytoplasm, cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 4133 Human
- Entrez Gene: 17756 Mouse
- Entrez Gene: 25595 Rat
- Omim: 157130 Human
- SwissProt: P11137 Human
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 368281 Human
see all -
Alternative names
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
Images
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This data was developed using ab281588, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling MAP2 with 281588 at 1/100 (5.52 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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This data was developed using ab281588, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling MAP2 with 281588 at 1/2000 (0.276 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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This data was developed using ab281588, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Mouse primary neuron cells labelling MAP2 with 281588 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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All lanes : Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588) at 1/1000 dilution
Lane 1 : SK-N-BE(2) (Human neuroblastoma neuroblast) whole cell lysate
Lane 2 : IMR-32 (Human neuroblastoma neuroblast) whole cell lysate
Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 199 kDa
Observed band size: 280,70 kDa why is the actual band size different from the predicted?This data was developed using ab281588, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.
Exposure time: 48 seconds.
-
This data was developed using ab281588, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling MAP2 with 281588 at 1/100 (5.52 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
All lanes : Anti-MAP2 antibody [RM1010] - Neuronal Marker (ab281588) at 1/1000 dilution
Lane 1 : Mouse E12.5 brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse cerebellum lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat cerebellum lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 199 kDa
Observed band size: 70-280 kDa why is the actual band size different from the predicted?This data was developed using ab281588, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.
Exposure time: 3 seconds.
-
This data was developed using ab281588, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling MAP2 with 281588 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab283865 has not yet been referenced specifically in any publications.