Overview

  • Product name

  • Description

    Rabbit polyclonal to MAP2K1IP1
  • Host species

    Rabbit
  • Specificity

    At least two isoforms of MAP2K1IP1 are known to exist; ab191581 will detect both isoforms. ab191581 is predicted to not cross-react with other LAMTOR family proteins.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MAP2K1IP1 (internal sequence). (15 amino acids). NP_068805.
    Database link: Q9UHA4

  • Positive control

    • Human brain tissue; Human brain tissue lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 14 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.
  • Sequence similarities

    Belongs to the LAMTOR3 family.
  • Cellular localization

    Late endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • LAMTOR3 antibody
    • Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3 antibody
    • LTOR3_HUMAN antibody
    • MAP2K1IP1 antibody
    • MEK binding partner 1 antibody
    • MEK partner 1 antibody
    • MEK-binding partner 1 antibody
    • Mitogen activated protein kinase kinase 1 interacting protein 1 antibody
    • Mitogen-activated protein kinase kinase 1-interacting protein 1 antibody
    • Mitogen-activated protein kinase scaffold protein 1 antibody
    • Mp1 antibody
    • Ragulator complex protein LAMTOR3 antibody
    see all

Images

  • Lane 1 : Anti-MAP2K1IP1 antibody (ab191581) at 1 µg/ml
    Lane 2 : Anti-MAP2K1IP1 antibody (ab191581) at 2 µg/ml

    All lanes : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 14 kDa

  • Immunofluorescent analysis of Human brain tissue labeling MAP2K1IP1 with ab191581 at 20 µg/ml.

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling MAP2K1IP1 with ab191581 at 5 µg/ml.

References

ab191581 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191581.
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