Key features and details
- Rabbit polyclonal to MAP2K1IP1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MAP2K1IP1 antibody
See all MAP2K1IP1 primary antibodies
DescriptionRabbit polyclonal to MAP2K1IP1
SpecificityAt least two isoforms of MAP2K1IP1 are known to exist; ab191581 will detect both isoforms. ab191581 is predicted to not cross-react with other LAMTOR family proteins.
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human MAP2K1IP1 (internal sequence). (15 amino acids). NP_068805.
Database link: Q9UHA4
- Human brain tissue; Human brain tissue lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 14 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionAs part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.
Sequence similaritiesBelongs to the LAMTOR3 family.
Cellular localizationLate endosome membrane.
- Information by UniProt
- LAMTOR3 antibody
- Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3 antibody
- LTOR3_HUMAN antibody
Lane 1 : Anti-MAP2K1IP1 antibody (ab191581) at 1 µg/ml
Lane 2 : Anti-MAP2K1IP1 antibody (ab191581) at 2 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 14 kDa
Immunofluorescent analysis of Human brain tissue labeling MAP2K1IP1 with ab191581 at 20 µg/ml.
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling MAP2K1IP1 with ab191581 at 5 µg/ml.
ab191581 has not yet been referenced specifically in any publications.