Overview

  • Product name

    Anti-MAP2K1IP1 antibody [Y130]
    See all MAP2K1IP1 primary antibodies
  • Description

    Rabbit monoclonal [Y130] to MAP2K1IP1
  • Host species

    Rabbit
  • Specificity

    ab32134 recognises MP1 (MEK partner-1).
  • Tested applications

    Suitable for: WB, IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C-terminus of human mitogen activated protein kinase kinase 1 interacting protein 1

  • Positive control

    • A431 cell lysate and human breast carcinoma
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    Y130
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 14 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
IP 1/50.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.
    • Sequence similarities

      Belongs to the LAMTOR3 family.
    • Cellular localization

      Late endosome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • LAMTOR3 antibody
      • Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3 antibody
      • LTOR3_HUMAN antibody
      • MAP2K1IP1 antibody
      • MEK binding partner 1 antibody
      • MEK partner 1 antibody
      • MEK-binding partner 1 antibody
      • Mitogen activated protein kinase kinase 1 interacting protein 1 antibody
      • Mitogen-activated protein kinase kinase 1-interacting protein 1 antibody
      • Mitogen-activated protein kinase scaffold protein 1 antibody
      • Mp1 antibody
      • Ragulator complex protein LAMTOR3 antibody
      see all

    Images

    • Anti-MAP2K1IP1 antibody [Y130] (ab32134) at 1/50000 dilution + A431 cell lysate

      Observed band size: 14 kDa
      why is the actual band size different from the predicted?

    • ab32134, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab32134 showing positive staining in Breast carcinoma tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Schiefermeier N  et al. The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration. J Cell Biol 205:525-540 (2014). IP ; Human . Read more (PubMed: 24841562) »
    • Westerman BA  et al. A genome-wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation. J Exp Med 208:2675-89 (2011). Read more (PubMed: 22143885) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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