Anti-MAP3K1 antibody [2F6] (ab233925)
Key features and details
- Mouse monoclonal [2F6] to MAP3K1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-MAP3K1 antibody [2F6]
See all MAP3K1 primary antibodies -
Description
Mouse monoclonal [2F6] to MAP3K1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human MAP3K1 aa 1050-1200.
Database link: Q13233 -
Positive control
- IHC-P: Human uterine carcinoma, thyroid carcinoma and cervical carcinoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
2F6 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at RT. |
Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Primary incubation for 30 minutes at RT. |
Target
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Function
Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 RING-type zinc finger.
Contains 1 SWIM-type zinc finger. -
Post-translational
modificationsAutophosphorylated. - Information by UniProt
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Database links
- Entrez Gene: 4214 Human
- Entrez Gene: 26401 Mouse
- Entrez Gene: 116667 Rat
- Omim: 600982 Human
- SwissProt: Q13233 Human
- SwissProt: P53349 Mouse
- SwissProt: Q62925 Rat
- Unigene: 653654 Human
see all -
Alternative names
- M3K1_HUMAN antibody
- MAP3K1 antibody
- MAPK/ERK kinase kinase 1 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human uterine carcinoma tissue stained for MAP3K1 using ab233925 at 1 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human thyroid carcinoma tissue stained for MAP3K1 using ab233925 at 1 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cervical carcinoma tissue stained for MAP3K1 using ab233925 at 1 μg/ml in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233925 has not yet been referenced specifically in any publications.