Product nameAnti-MAP3K1 (phospho T1400) antibody
See all MAP3K1 primary antibodies
DescriptionRabbit polyclonal to MAP3K1 (phospho T1400)
ab138662 detects endogenous levels of MAP3K1 only when phosphorylated at T1400 in Human samples or at T1381 in Mouse samples.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human MAP3K1 (internal sequence). Immunogen derived a synthesized phospho-peptide from human MAP3K1 around the phosphorylation site of Thr1400.
Database link: Q13233
- Extracts from Jurkat cells
This product was previously labelled as MEKK1
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab138662 was purified from Rabbit antiserum. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation.
Our Abpromise guarantee covers the use of ab138662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 164 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 RING-type zinc finger.
Contains 1 SWIM-type zinc finger.
- Information by UniProt
- M3K1_HUMAN antibody
- MAP3K1 antibody
- MAPK/ERK kinase kinase 1 antibody
Anti-MAP3K1 (phospho T1400) antibody (ab138662) at 1/500 dilution + extracts from Jurkat cells at 30 µg
Predicted band size: 164 kDa
All lanes : Anti-MAP3K1 (phospho T1400) antibody (ab138662) at 1/250 dilution
Lane 1 : Mouse B16F10 whole cell lysate - untreated
Lane 2 : Mouse B16F10 whole cell lysate - treated with 5µM Osteoponin for 10 minutes
Lysates/proteins at 60 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 164 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
This product has been referenced in:
- Chen ZS et al. Planar cell polarity gene Fuz triggers apoptosis in neurodegenerative disease models. EMBO Rep 19:N/A (2018). Read more (PubMed: 30026307) »
- Saha K et al. p38d regulates p53 to control p21Cip1 expression in human epidermal keratinocytes. J Biol Chem 289:11443-53 (2014). WB . Read more (PubMed: 24599959) »