Overview

  • Product name

    Anti-MAP3K1 (phospho T1400) antibody
    See all MAP3K1 primary antibodies
  • Description

    Rabbit polyclonal to MAP3K1 (phospho T1400)
  • Host species

    Rabbit
  • Specificity

    ab138662 detects endogenous levels of MAP3K1 only when phosphorylated at T1400 in Human samples or at T1381 in Mouse samples.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human MAP3K1 (internal sequence). Immunogen derived a synthesized phospho-peptide from human MAP3K1 around the phosphorylation site of Thr1400.
    Database link: Q13233

  • Positive control

    • Extracts from Jurkat cells
  • General notes

     This product was previously labelled as MEKK1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab138662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 164 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 RING-type zinc finger.
    Contains 1 SWIM-type zinc finger.
  • Post-translational
    modifications

    Autophosphorylated.
  • Information by UniProt
  • Database links

  • Alternative names

    • M3K1_HUMAN antibody
    • MAP3K1 antibody
    • MAPK/ERK kinase kinase 1 antibody
    • MAPKKK1 antibody
    • MEK kinase 1 antibody
    • MEKK 1 antibody
    • Mekk antibody
    • MEKK1 antibody
    • Mitogen activated protein kinase kinase kinase 1 antibody
    • Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase antibody
    • Mitogen-activated protein kinase kinase kinase 1 antibody
    • SRXY6 antibody
    see all

Images

  • Anti-MAP3K1 (phospho T1400) antibody (ab138662) at 1/500 dilution + extracts from Jurkat cells at 30 µg

    Predicted band size: 164 kDa

  • All lanes : Anti-MAP3K1 (phospho T1400) antibody (ab138662) at 1/250 dilution

    Lane 1 : Mouse B16F10 whole cell lysate - untreated
    Lane 2 : Mouse B16F10 whole cell lysate - treated with 5µM Osteoponin for 10 minutes

    Lysates/proteins at 60 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 164 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes

    See Abreview

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.1% saponin
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 09 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (7.5)
Loading amount
0.2 µg
Specification
HeLa
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 02 2019

Application
Western blot
Sample
Mouse Cell lysate - whole cell (B16F10)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
60 µg
Treatment
5uM Osteopontin (OPN) for 10 minutes
Specification
B16F10
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 15 2014

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