Anti-MAP3K13 antibody (ab224628)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-MAP3K13 antibody
See all MAP3K13 primary antibodies -
Description
Rabbit polyclonal to MAP3K13 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human MAP3K13 aa 506-835.
Sequence:AVEKKYPGTYKRHPVRPIIHPNAMEKLMKRKGVPHKSGMQTKRPDLLRSE GIPTTEVAPTASPLSGSPKMSTSSSKSRYRSKPRHRRGNSRGSHSDFAAI LKNQPAQENSPHPTYLHQAQSQYPSLHHHNSLQQQYQQPPPAMSQSHHPR LNMHGQDIATCANNLRYFGPAAALRSPLSNHAQRQLPGSSPDLISTAMAA DCWRSSEPDKGQAGPWGCCQADAYDPCLQCRPEQYGSLDIPSAEPVGRSP DLSKSPAHNPLLENAQSSEKTEENEFSGCRSESSLGTSHLGTPPALPRKT RPLQKSGDDSSEEEEGEVDSEVEFPRRQRP
Database link: O43283 -
Positive control
- WB: Rat liver and lung tissue lysates; Mouse liver and stomach tissue lysates. IHC-P: Human brain tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab224628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/5000. Predicted molecular weight: 14,85,108 kDa. | |
| IHC-P | 1/20 - 1/200. |
Target
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Function
Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B. -
Tissue specificity
Expressed in the adult brain, liver, placenta and pancreas, with expression strongest in the pancreas. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated on serine and threonine residues. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 9175 Human
- Entrez Gene: 71751 Mouse
- Entrez Gene: 303823 Rat
- Omim: 604915 Human
- SwissProt: O43283 Human
- SwissProt: Q1HKZ5 Mouse
- Unigene: 634586 Human
- Unigene: 656069 Human
see all -
Alternative names
- EC 2.7.11.25 antibody
- Leucine zipper bearing kinase antibody
- Leucine zipper-bearing kinase antibody
see all
Images
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Western blot - Anti-MAP3K13 antibody (ab224628)All lanes : Anti-MAP3K13 antibody (ab224628) at 1/500 dilution
Lane 1 : Rat liver tissue lysate
Lane 2 : Rat lung tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Mouse stomach tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14,85,108 kDa
Observed band size: 109 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP3K13 antibody (ab224628)Paraffin-embedded human brain tissue stained for MAP3K13 using ab224628 at 1/100 dilution in immunohistochemical analysis.
References
ab224628 has not yet been referenced specifically in any publications.
