Immunohistochemistry (Frozen sections) abreview for Anti-MAP3K15 antibody - N-terminal

Inconclusive
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - 30min in citrate buffer @ 90oC
Specification
Brain
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Other product details

Incubation time
30 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 2% normal goat serum
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG biotinylated
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
The literature in the mouse shows that MAP3K15 specifically stains the CA2 region of the hippocampus as well as other non-hippocampal regions. This was not found in the rat using this antibody. There apeared to be some staining throughout the pyramidal layer in all regions of the hippocampus. It could however be background staining. It is unclear. It is likely that the problem is due to the antibody. There was high background staining and much of the neuronal staining seen in other regions could be due to background. The alternative is that MAP3K15 does not specifically stain the CA2 in rat, although this is very unlikely.
Abcam response
Thank you so much for testing this antibody in rat and IHC-Fr. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species and application. We welcome additional feedback from other customers as well.

Dr. Emma Schofield

Verified customer

Submitted Sep 15 2016

Sign up