Recombinant Anti-MAP3K4 antibody [EP551Y] (ab40784)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP551Y] to MAP3K4
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MAP3K4 antibody [EP551Y]
See all MAP3K4 primary antibodies -
Description
Rabbit monoclonal [EP551Y] to MAP3K4 -
Host species
Rabbit -
Specificity
This antibody is predicted to detect splice isoform 2 based on sequence analysis. -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MAP3K4 aa 150-250.
Database link: Q9Y6R4 -
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP551Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 182 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 182 kDa). |
Target
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Function
Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6. -
Tissue specificity
Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm > perinuclear region. Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles. - Information by UniProt
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Database links
- Entrez Gene: 4216 Human
- Omim: 602425 Human
- SwissProt: Q9Y6R4 Human
- Unigene: 390428 Human
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Alternative names
- dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4) antibody
- FLJ42439 antibody
- KIAA0213 antibody
see all
Images
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Anti-MAP3K4 antibody [EP551Y] (ab40784) at 1/1000 dilution + 293 cell lysate. at 10 µg
Predicted band size: 182 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded human ovary adenocarcinoma using ab40784.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab40784 has been referenced in 1 publication.
- Duan JL et al. A novel peptide encoded by N6-methyladenosine modified circMAP3K4 prevents apoptosis in hepatocellular carcinoma. Mol Cancer 21:93 (2022). PubMed: 35366894