Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP551Y] to MAP3K4
- Suitable for: IHC-P, ICC, Flow Cyt, WB
- Reacts with: Human
Product nameAnti-MAP3K4 antibody [EP551Y]
See all MAP3K4 primary antibodies
DescriptionRabbit monoclonal [EP551Y] to MAP3K4
SpecificityThis antibody is predicted to detect splice isoform 2 based on sequence analysis.
Tested applicationsSuitable for: IHC-P, ICC, Flow Cyt, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human MAP3K4 aa 150-250.
Database link: Q9Y6R4
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab40784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 182 kDa).|
FunctionComponent of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.
Tissue specificityExpressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm > perinuclear region. Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles.
- Information by UniProt
- dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4) antibody
- FLJ42439 antibody
- KIAA0213 antibody
Anti-MAP3K4 antibody [EP551Y] (ab40784) at 1/1000 dilution + 293 cell lysate. at 10 µg
Predicted band size: 182 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded human ovary adenocarcinoma using ab40784.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab40784 has not yet been referenced specifically in any publications.