Overview

  • Product name

  • Description

    Rabbit polyclonal to MAP3K8/COT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human MAP3K8/COT aa 223-467.
    Database link: P41279

  • Positive control

    • Recombinant Human MAP3K8/COT protein (ab132532) can be used as a positive control in WB. A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; Human NCIN87 xenograft tissue; HeLa cells.
  • General notes

     This product was previously labelled as MAP3K8

     

Properties

Applications

Our Abpromise guarantee covers the use of ab137589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.
  • Tissue specificity

    Expressed in several normal tissues and human tumor-derived cell lines.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Developmental stage

    Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle.
  • Post-translational
    modifications

    Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AURA2 antibody
    • c COT antibody
    • Cancer Osaka thyroid oncogene antibody
    • CCOT antibody
    • COT antibody
    • COT proto oncogene serine/threonine protein kinase antibody
    • EST antibody
    • ESTF antibody
    • Ewing sarcoma transformant antibody
    • FLJ10486 antibody
    • M3K8_HUMAN antibody
    • MAP3K 8 antibody
    • MAP3K8 antibody
    • MEKK8 antibody
    • Mitogen activated protein kinase kinase kinase 8 antibody
    • Mitogen-activated protein kinase kinase kinase 8 antibody
    • Proto oncogene cCot antibody
    • Proto-oncogene c-Cot antibody
    • Serine/threonine protein kinase cot antibody
    • Serine/threonine-protein kinase cot antibody
    • TPL 2 antibody
    • TPL-2 antibody
    • TPL2 antibody
    • Tumor progression locus 2 antibody
    see all

Images

  • Anti-MAP3K8/COT antibody (ab137589) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 52 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue, labelling MAP3K8/COT with ab137589 at 1/500 dilution.

  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling MAP3K8/COT with ab137589 at 1/200 dilution. The lower image is co-stained with Hoechst 33342.

References

This product has been referenced in:

  • Chorzalska A  et al. Overexpression of Tpl2 is linked to imatinib resistance and activation of MEK-ERK and NF-?B pathways in a model of chronic myeloid leukemia. Mol Oncol N/A:N/A (2018). WB . Read more (PubMed: 29485707) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (macrophage)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 12 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up