Anti-MAP3K8/COT antibody (ab196751)
Key features and details
- Rabbit polyclonal to MAP3K8/COT
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAP3K8/COT antibody
See all MAP3K8/COT primary antibodies -
Description
Rabbit polyclonal to MAP3K8/COT -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human MAP3K8/COT (C terminal). The exact sequence is proprietary. [The non-phosphopeptide was derived from around the phosphorylation site of serine 400 (C-Q-S(p)-L-D).]
Database link: P41279 -
Positive control
- Insulin treated COLO cell lysate; Human brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS
PBS is without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab196751 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100.
|
Notes |
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IHC-P
1/50 - 1/100. |
Target
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Function
Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. -
Tissue specificity
Expressed in several normal tissues and human tumor-derived cell lines. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. -
Developmental stage
Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle. -
Post-translational
modificationsAutophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1326 Human
- Entrez Gene: 26410 Mouse
- Entrez Gene: 116596 Rat
- Omim: 191195 Human
- SwissProt: P41279 Human
- SwissProt: Q07174 Mouse
- SwissProt: Q63562 Rat
- Unigene: 432453 Human
see all -
Alternative names
- AURA2 antibody
- c COT antibody
- Cancer Osaka thyroid oncogene antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196751 has not yet been referenced specifically in any publications.