Key features and details
- Rabbit polyclonal to MAP3K8/COT
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAP3K8/COT antibody
See all MAP3K8/COT primary antibodies
DescriptionRabbit polyclonal to MAP3K8/COT
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human MAP3K8/COT (C terminal). The exact sequence is proprietary. [The non-phosphopeptide was derived from around the phosphorylation site of serine 400 (C-Q-S(p)-L-D).]
Database link: P41279
- Insulin treated COLO cell lysate; Human brain tissue.
This product was previously labelled as MAP3K8
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS
PBS is without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab196751 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab196751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
FunctionRequired for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.
Tissue specificityExpressed in several normal tissues and human tumor-derived cell lines.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Developmental stageIsoform 1 is activated specifically during the S and G2/M phases of the cell cycle.
modificationsAutophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.
- Information by UniProt
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ab196751 has not yet been referenced specifically in any publications.