Key features and details
- Rabbit polyclonal to MAP3K8/COT
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MAP3K8/COT antibody
See all MAP3K8/COT primary antibodies
DescriptionRabbit polyclonal to MAP3K8/COT
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
This product was previously labelled as MAP3K8
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 52 kDa.|
FunctionRequired for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.
Tissue specificityExpressed in several normal tissues and human tumor-derived cell lines.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Developmental stageIsoform 1 is activated specifically during the S and G2/M phases of the cell cycle.
modificationsAutophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.
- Information by UniProt
- AURA2 antibody
- c COT antibody
- Cancer Osaka thyroid oncogene antibody
All lanes : Anti-MAP3K8/COT antibody (ab217684) at 1/200 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 52 kDa
12% gel. Incubation with ab217684 done at 4°C. Conjugation to secondary antibody done for 90min at 37°C.
ab217684 has not yet been referenced specifically in any publications.