• Product name

    Anti-MAP3K8/COT antibody [EP1103Y]
    See all MAP3K8/COT primary antibodies
  • Description

    Rabbit monoclonal [EP1103Y] to MAP3K8/COT
  • Host species

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MAP3K8/COT aa 350-450. The exact sequence is proprietary.

  • Positive control

    • Jurkat whole cell lysate (ab7899)
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


     This product was previously labelled as MAP3K8


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab52613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 53 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.
    • Tissue specificity

      Expressed in several normal tissues and human tumor-derived cell lines.
    • Sequence similarities

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Developmental stage

      Isoform 1 is activated specifically during the S and G2/M phases of the cell cycle.
    • Post-translational

      Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • AURA2 antibody
      • c COT antibody
      • Cancer Osaka thyroid oncogene antibody
      • CCOT antibody
      • COT antibody
      • COT proto oncogene serine/threonine protein kinase antibody
      • EST antibody
      • ESTF antibody
      • Ewing sarcoma transformant antibody
      • FLJ10486 antibody
      • M3K8_HUMAN antibody
      • MAP3K 8 antibody
      • MAP3K8 antibody
      • MEKK8 antibody
      • Mitogen activated protein kinase kinase kinase 8 antibody
      • Mitogen-activated protein kinase kinase kinase 8 antibody
      • Proto oncogene cCot antibody
      • Proto-oncogene c-Cot antibody
      • Serine/threonine protein kinase cot antibody
      • Serine/threonine-protein kinase cot antibody
      • TPL 2 antibody
      • TPL-2 antibody
      • TPL2 antibody
      • Tumor progression locus 2 antibody
      see all


    • Anti-MAP3K8/COT antibody [EP1103Y] (ab52613) at 1/500 dilution + Jurkat cell lysate at 10 µg

      Goat anti-rabbit HRP antibody at 1/2000 dilution

      Predicted band size: 53 kDa
      Observed band size: 53 kDa


    ab52613 has not yet been referenced specifically in any publications.

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    There are currently no Customer reviews or Questions for ab52613.
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